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2004 | 51 | 3 | 851-856
Article title

Antitumour activity of Salmonella typhimurium VNP20047 in B16(F10) murine melanoma model.

Content
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EN
Abstracts
EN
A tumour therapy is proposed based on attenuated Salmonella typhimurium VNP20047 expressing the Escherichia coli cytosine deaminase gene. VNP20047 was administered intravenously to B16(F10) melanoma-bearing C57BL/6 mice. VNP20047 proliferated within tumours and livers regardless of the initial inoculum dose. After 10 days the number of bacteria increased in livers up to 4.2 × 106 cfu/g and decreased in tumours down to 5.9 × 106 cfu/g. VNP20047 at 1 × 105 cfu/mouse, when combined with 5-fluorocytosine, inhibited tumour growth by 85% without prolonging animal survival. Histology studies revealed severe lesions in tumours and livers. These data suggest that S. typhimurium VNP20047 induced inflammatory responses, even though the strain was attenuated.
Publisher

Year
Volume
51
Issue
3
Pages
851-856
Physical description
Dates
published
2004
received
2004-03-02
revised
2004-04-23
accepted
2004-04-27
Contributors
  • Department of Molecular Biology, Centre of Oncology, Maria Skłodowska-Curie Memorial Institute, Gliwice, Poland
  • Department of Molecular Biology, Centre of Oncology, Maria Skłodowska-Curie Memorial Institute, Gliwice, Poland
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv51i3p851kz
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