Thiamine prevents X-ray induction of genetic changes in human lymphocytes in vitro.

• Authors: Maria Konopacka , Jacek Rogoliński
• Languages of publication: EN

Abstracts

EN

The effects of thiamine (vitamin B1) on the level of spontaneous or radiation-induced genetic changes in human lymphocytes in vitro were studied. Cultured lymphocytes were exposed to increasing concentrations of thiamine (0-500 μg/ml) and irradiated with X-rays. The DNA damage was estimated as the frequency of micronuclei and apoptotic or necrotic morphological changes in fixed cells. The results show that thiamine alone did not induce genetic changes. A significant decrease in the fraction of apoptotic and necrotic cells was observed in lymphocytes irradiated in the presence of vitamin B1 at concentrations between 1-100 μg/ml compared to those irradiated in the absence of thiamine. Vitamin B1 at 1 and 10 μg/ml decreased also the extent of radiation-induced formation of micronuclei. Vitamin B1 had no effect on radiation-induced cytotoxicity as measured by nuclear division index. The results indicate that vitamin B1 protects human cells from radiation-induced genetic changes.

Keywords

EN

thiamine; X-radiation; necrosis; human lymphocytes; micronuclei; apoptosis

• Journal: Acta Biochimica Polonica
• Year: 2004
• Volume: 51
• Issue: 3
• Pages: 839-843

Dates

published

2004

received

2004-03-02

revised

2004-06-23

accepted

2004-07-07

Contributors

author

Maria Konopacka

• Department of Experimental and Clinical Radiobiology, Institute of Oncology, Gliwice, Poland

author

Jacek Rogoliński

• Department of Experimental and Clinical Radiobiology, Institute of Oncology, Gliwice, Poland

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