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2004 | 51 | 3 | 839-843
Article title

Thiamine prevents X-ray induction of genetic changes in human lymphocytes in vitro.

Content
Title variants
Languages of publication
EN
Abstracts
EN
The effects of thiamine (vitamin B1) on the level of spontaneous or radiation-induced genetic changes in human lymphocytes in vitro were studied. Cultured lymphocytes were exposed to increasing concentrations of thiamine (0-500 μg/ml) and irradiated with X-rays. The DNA damage was estimated as the frequency of micronuclei and apoptotic or necrotic morphological changes in fixed cells. The results show that thiamine alone did not induce genetic changes. A significant decrease in the fraction of apoptotic and necrotic cells was observed in lymphocytes irradiated in the presence of vitamin B1 at concentrations between 1-100 μg/ml compared to those irradiated in the absence of thiamine. Vitamin B1 at 1 and 10 μg/ml decreased also the extent of radiation-induced formation of micronuclei. Vitamin B1 had no effect on radiation-induced cytotoxicity as measured by nuclear division index. The results indicate that vitamin B1 protects human cells from radiation-induced genetic changes.
Year
Volume
51
Issue
3
Pages
839-843
Physical description
Dates
published
2004
received
2004-03-02
revised
2004-06-23
accepted
2004-07-07
References
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv51i3p839kz
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