PL EN


Preferences help
enabled [disable] Abstract
Number of results
2004 | 51 | 3 | 773-788
Article title

Involvement of Na+/H+ exchanger in desmopressin-induced platelet procoagulant response.

Content
Title variants
Languages of publication
EN
Abstracts
EN
Desmopressin (DDAVP) action on platelets is associated with the development of procoagulant response but the underlying mechanism of this phenomenon is not known. We investigated whether this effect of DDAVP might be due to activation of plasma membrane Na+/H+ exchanger. The DDAVP-induced platelet procoagulant response, measured as phospholipid-dependent thrombin generation, was dose dependent and significantly weaker than that produced by collagen or monensin (mimics Na+/H+ antiport). Both the DDAVP- and collagen-produced procoagulant responses were less pronounced in the presence of EIPA, an Na+/H+ exchanger inhibitor. Flow cytometry studies revealed that in vitro treatment of platelets with DDAVP or collagen was associated with the appearance of both degranulated (and fragmented) and swollen cells. The DDAVP-evoked rise in size and granularity heterogeneity was similar to that produced by collagen or monensin and was not observed in the presence of EIPA. Using flow cytometry and annexin V-FITC as a probe for phosphatidylserine (PS) we demonstrated increased and uniform binding of this marker to all subsets of DDAVP-treated platelet population. The DDAVP-evoked PS expression was dose dependent, strongly reduced by EIPA and weaker than that caused by monensin or collagen. As judged by optical swelling assay, DDAVP in a dose dependent manner produced a rise in platelet volume. The swelling was inhibited by EIPA and its kinetics was similar to that observed in the presence of monensin. Electronic cell-sizing measurements showed an increase in mean platelet volume and a decrease in platelet count and platelet crit upon treatment with DDAVP. DDAVP elicited a slow (much slower than collagen) alkalinization of platelet cytosol. Altogether the data indicate an involvement of Na+/H+ exchanger in the generation of procoagulant activity in DDAVP-treated platelets.
Publisher

Year
Volume
51
Issue
3
Pages
773-788
Physical description
Dates
published
2004
received
2004-02-24
revised
2004-05-11
accepted
2004-07-08
Contributors
  • Department of Allergology and Internal Diseases, Medical University of Białystok, Białystok, Poland
  • Department of Physical Chemistry, Medical University of Białystok, Białystok, Poland
  • Department of Allergology and Internal Diseases, Medical University of Białystok, Białystok, Poland
  • Department of Physical Chemistry, Medical University of Białystok, Białystok, Poland
References
  • Aharonovitz O, Granot Y. (1996) Stimulation of mitogen-activated protein kinase and Na+/H+ exchanger in human platelets. Differential effects of phorbol ester and vasopressin. J Biol Chem.; 271: 16494-9.
  • Apodaca G. (2002) Modulation of membrane traffic by mechanical stimuli. Am J Physiol Renal Physiol.; 282: F179-0.
  • Balduini CL, Noris P, Belletti S, Spedini P, Gamba G. (1999) In vitro and in vivo effect of desmopressin on platelet function. Haematologica.; 84: 891-6.
  • Bertino AM, Qi XO, Li J, Xia Y, Kuter DJ. (2003) Apoptotic markers are increased in platelets stored at 37°C. Transfusion.; 43: 857-66.
  • Bode AP, Hickerson DH. (2000) Characterization and quantitation by flow cytometry of membranous microparticles formed during activation of platelet suspensions with ionophore or thrombin. Platelets.; 11: 259-71.
  • Borin M, Siffert W. (1990) Stimulation by thrombin increases the cytosolic free Na+ concentration in human platelets. Studies with the novel fluorescent cytosolic Na+ indicator sodium-binding benzofuran isophtalate. J Biol Chem.; 265: 19543-50.
  • Borin M, Siffert W. (1991) Further characterization of the mechanisms mediating the rise in cytosolic free Na+ in thrombin-stimulated platelets. Evidence for inhibition of the Na+, K+-ATPase and for Na+ entry via a Ca2+ influx pathway. J Biol Chem.; 266: 13153-60.
  • Bruzgo I, Tomasiak M, Stelmach H, Midura-Nowaczek K. (2004) The effect of some epsilon-aminocaproic acid derivatives on platelet responses. Acta Biochim Polon.; 51: 73-80.
  • Castaman G, Rodheghiero F, Lattuado A, Mannuci P. (1993) Desmopressin-induced thrombocytopenia in type I platelet disordant von Willebrand disease. Am J Hematol.; 43: 5-9.
  • Cattaneo M, Moia M, Delle Valle P, Castellana P, Mannucci PM. (1989) DDAVP shortens the prolonged bleeding times of patients with severe von Willebrand disease treated with cryoprecipitate. Evidence for a mechanism of action independent of released von Willebrand factor. Blood.; 74: 1972-5.
  • Dimichele DM, Hathaway WE. (1990) Use of DDAVP in inherited and acquired platelet dysfunction. Am J Hematol.; 33: 39-45.
  • Ghosh S, Rao AK. (1993) Lack of effect of 1-deamino-8-D-arginine vasopressin on direct adhesion to collagen. Thromb Res.; 70: 417-22.
  • Giorgi PL, Fiorini R, Biraghi M, Kantar A. (1996) Platelet plasma membrane in subjects with primary nocturnal enuresis: effect of desmopressin. Biol Cell.; 86: 135-7.
  • Greinacher A, Potzssch B, Kiefel V, White JG, Muller-Berghaus G, Mueller-Eckhardt C. (1993) Evidence that DDAVP transiently improves hemostasis in Bernard-Soulier syndrome independent of von Willebrand-factor. Ann Hematol.; 67: 149-50.
  • Hacker G. (2000) The morphology of apoptosis. Cell Tissue Res.; 301: 5-17.
  • Hamill OP, Martinac B. (2001) Molecular basis of mechanotransduction in living cells. Physiol Rev.; 81: 685-729.
  • Heemskerk J, Bevers E, Lindhout T. (2002) Platelet activation and blood coagulation. Thromb Haemost.; 88: 186-93.
  • Horstman LL, Valle-Riestra BJ, Jy W, Wang F, Mao W, Ahn Y. (1995) Desmopressin (DDAVP) acts on platelets to generate platelet microparticles and enhanced procoagulant activity. Thromb Res.; 79: 163-174.
  • Johnstone MT, Andrews T, Ware JA, Rudd MA, George D, Weinstein M, Loscalzo J. (1990) Bleeding time prolongation with streptokinase and its reduction with 1-desamino-8-D- arginine vasopressin. Circulation.; 82: 2142-51.
  • Jy W, Horstman LL, Park H, Mao WW, Valant P, Ahn YS. (1998) Platelet aggregates as markers of platelet activation: characterization of flow cytometric method suitable for clinical applications. Am J Hematol.; 57: 33-42.
  • Lethagen S. (2003) Desmopressin in mild hemophilia A: indications, limitations, efficacy, and safety. Semin Thromb Hemost.; 29: 101-5.
  • Li J, Xia Y, Bertino A, Coburn JP, Kuter DJ. (2000) The mechanism of apoptosis in human platelets during storage. Transfusion.; 40: 1320-9.
  • Mannucci M. (1997) Desmopressin (DDAVP) in the treatment of bleeding disorders: the first 20 years. Blood.; 90: 2515-21.
  • Martin SJ, Reutelingsperger CPM, McGahon AJ, Rader JA, van Schie RC, LaFace DM, Green DR. (1995) Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl. J Exp Med.; 182: 1545-56.
  • Orlowski J, Grinstein S. (1997) Na+/H+ exchangers of mammalian cells. J Biol Chem.; 272: 22373-6.
  • Reed G, Fitzgerald M, Polgar J. (2000) Molecular mechanisms of platelet exocytosis: insights into the secrete life of thrombocytes. Blood.; 96: 3334-42.
  • Reiter RA, Mayr F, Blazicek H, Galehr E, Jilma-Stohlavetz P, Domanovits H, Jilma B. (2003) Desmopressin and platelets antagonize the in vitro platelet dysfunction induced by GP IIb/IIIa inhibitors and aspirin. Blood.; 102: 4594-9.
  • Rosskopf D. (1999) Sodium-hydrogen exchange and platelet function. J Thromb Thrombolysis.; 8: 15-23.
  • Rosskopf D, Morgenstern E, Scholz W, Osswald U, Siffert W. (1991) Rapid determination of the elevated Na+ /H+ exchange in platelets of patients with essential hypertension using an optical swelling assay. J Hypertens.; 9: 231-8.
  • Rota S, Flynn PD, Wareham NJ, Baglin TP. (1996) Is platelet phospholipid-dependent thrombin generation altered by acute myocardial infarction or aspirin? Thromb Res.; 83: 329-38.
  • Samson J, Stelmach H, Tomasiak M. (2001) The importance of Na+/H+ exchanger for the generation of procoagulant activity by porcine blood platelets. Platelets.; 12: 436-42.
  • Schulman S, Johnsson H. (1991) Heparin, DDAVP and the bleeding time. Thromb Haemost.; 65: 242-4.
  • Shapira S, Friedman Z, Shapiro H, Presseizen K, Radnay J, Ells MH. (2000) The effect of storage on the expression of platelet membrane phosphatidylserine and the subsequent impact on the coagulant function of stored platelets. Transfusion.; 40: 1257-63.
  • Siffert W, Akkerman JWN. (1988) Na+/H+ exchange as a modulator of platelet activation. Trends Biochem Sci.; 13: 148-51.
  • Siffert W, Siffert G, Scheid P, Akkerman JWN. (1989) Activation of Na+/H+ exchange and Ca2+ mobilization start simultaneously in thrombin-stimulated platelets. Evidence that platelet shape change disturbs early rises of BCECF fluorescence which causes an underestimation of actual cytosolic alkalinization. Biochem J.; 258: 521-7.
  • Sims PJ, Wiedmer T. (2001) Unraveling the mysteries of phospholipid scrambling. Thromb Haemost.; 86: 266-75.
  • Stelmach H, Rusak T, Tomasiak M. (2002) The involvement of the Na+/H+ exchanger in the formation of microvesicles by porcine blood platelets. Haematologia.; 32: 239-52.
  • Sweatt JD, Johnson SL, Cragoe EJ, Limbird LE. (1985) Inhibitors of Na+/H+ exchange block stimulus-provoked arachidonic acid release in human platelets. Selective effects on platelet activation by epinephrine, ADP and lower concentrations of thrombin. J Biol Chem.; 260: 12910-9.
  • Tomasiak MM, Stelmach H, Bodzenta A, Tomasiak M. (2003) The involvement of Na+/H+ exchanger in the desmopressin-induced platelet procoagulant response. 39th Meeting of the Polish Biochemical Society. Acta Biochim Polon.; 50: 201.
  • van Engeland M, Nieland LJ, Ramaekers FC, Schutte B, Reutelingsperger CP. (1998) Annexin V-affinity assay; a review on an apoptosis detection system based on phosphatidylserine exposure. Cytometry.; 31: 1-9.
  • Wun T, Paglieroni TG, Lachant NA. (1995) Desmopressin stimulates the expression of P-selectin on human platelets in vitro. J Lab Clin Med.; 126: 401-9.
  • Yang X, Disa J, Rao AK. (1990) Effect of 1-desamino-8-D-arginine vasopressin (DDAVP) on human platelets. Thromb Res.; 59: 809-18.
  • Zwaal RFA, Schroit AJ. (1997) Pathophysiologic implications of membrane phospholipid asymmetry in blood cells. Blood.; 89: 1121-32.
  • Zwaal R, Comfurius P, Bevers EM. (1998) Lipid-protein interactions in blood coagulation. Biochim Biophys Acta.; 1376: 433-53.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv51i3p773kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.