Restriction analysis of genetic variability of Polish isolates of Tomato black ring virus.

• Authors: Magdalena Jończyk , Natasza Borodynko , Henryk Pospieszny
• Languages of publication: EN

Abstracts

EN

Several different isolates of Tomato black ring virus (TBRV) have been collected in Poland from cucumber, tomato, potato and black locust plants. Biological tests showed some differences in the range of infected plants and the type of symptoms, which was the basis for selection of seven the most biologically different TBRV isolates. According to the sequence of TBRV-MJ, several primer pairs were designed and almost the entire sequence of both genomic RNAs was amplified. The RT-PCR products derived from all tested TBRV isolates were digested by restriction enzymes. On the basis of the restriction patterns, the variable and the conserved regions of the TBRV genome were defined and the relationships between the Polish TBRV isolates established.

Keywords

EN

restriction analysis; genetic variability; phylogeny; Tomato black ring virus

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2004
• Volume: 51
• Issue: 3
• Pages: 673-681

Dates

published

2004

received

2003-08-04

revised

2004-04-15

accepted

2004-04-23

Contributors

author

Magdalena Jończyk

• Department of Virology and Bacteriology, Institute of Plant Protection, Poznań, Poland

author

Natasza Borodynko

• Department of Virology and Bacteriology, Institute of Plant Protection, Poznań, Poland

author

Henryk Pospieszny

• Department of Virology and Bacteriology, Institute of Plant Protection, Poznań, Poland

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