Preferences help
enabled [disable] Abstract
Number of results
2004 | 51 | 3 | 625-634
Article title

Novel oligosaccharides isolated from Fusarium oxysporum L. rapidly induce PAL activity in Rubus cells.

Title variants
Languages of publication
Activation of the phenolic pathway is known to be part of a defense response against cell wall-derived elicitors from pathogens. Many examples of a defense response by increasing the synthesis of phenolic compound against the elicitor were demonstrated in the past, but the elicitor structure has so far been poorly characterized. Our results indicate that a disaccharide fraction containing the following structure: α-D-mannopyranosyl (1→2)α/β-D-glucopyranosyl and α-D-mannopyranosyl (1→x) inositol, isolated from Fusarium oxysporum L., promotes rapid and transient phenylalanine ammonia lyase activity in Rubus fructicosus cells at nanomolar concentration. The disaccharides were isolated by size-exclusion chromatography directly from extracts obtained by alkaline treatment of F. oxysporum mycelium. Their structure was determined by 500-MHz-1H-NMR spectroscopy combined with methylation analysis and fast atom bombardment mass spectrometry.
Physical description
  • Centre de Recherches sur les Macromolécules Végétales (CERMAV)-CNRS, Grenoble, France
  • Centre de Recherches sur les Macromolécules Végétales (CERMAV)-CNRS, Grenoble, France
  • Centre de Recherches sur les Macromolécules Végétales (CERMAV)-CNRS, Grenoble, France
  • Centre de Recherches sur les Macromolécules Végétales (CERMAV)-CNRS, Grenoble, France
  • Centre de Recherches sur les Macromolécules Végétales (CERMAV)-CNRS, Grenoble, France
  • Alconada TM, Martinez MJ. (1995) Isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from Fusarium oxysporum f.sp. melons. Rev Argent Microbiol.; 27: 191-7 (in Spanish).
  • Backman KS, Roberts M, Patterson R. (1995) Allergic bronchopulmonary mycosis caused by Fusarium vasinfectum. Am J Respir Crit Care Med.; 152: 1379-81.
  • Benhamou N, Chamberland H, Pauze FJ. (1990) Implication of pectic components in cell surface interactions between tomato root cells and Fusarium oxysporum f.sp. radicis- lycopersici. Plant Physiol.; 92: 995-1003.
  • Bradford MM. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem.; 72: 248-54.
  • Bruckner B, Blechschmidt D, Schubert B. (1989) Fusarium moniliforme Sheld, a fungus producing a broad spectrum of bioactive metabolites. Zentralbl Mikrobiol.; 144: 3-12.
  • Bruneteau M, Perret J, Rouhier P, Michel G. (1992) Structure of beta-D-glucans from Fusarium oxysporum. Carbohydr Res.; 236: 345-8.
  • Casacuberta JM, Raventos D, Puigdomenech P, San Segundo B. (1992) Expression of the gene encoding the PR-like protein PRms in germinating maize embryos. Mol Gen Genet.; 234: 97-104.
  • Christakopoulos P, Nerinck W, Krkos D, Macris B, Claeyssens M. (1996) Purification and characterization of two low molecular mass alkaline xylanases from Fusarium oxysporum F3. J Biotechnol.; 51: 181-9.
  • Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. (1956) Colorimetric method for determination of sugars and related substances. Anal Chem.; 28: 350-6.
  • El-Gendy W, Brownleader MD, Ismail H, Clarke PJ, Gilbert J, El-Bordiny F, Trevan M, Hopkins J, Naldrett M, Jackson P. (2001) Rapid deposition of wheat cell wall structural proteins in response to Fusarium-derived elicitors. J Exp Bot.; 52: 85-90.
  • Hagendoorn MJM, Poortinga AM, Sang HW, van der Plas LH, van Walraven HS. (1991) Effect of elicitor on the plasma membrane of Petunia hybrida cell suspensions. Plant Physiol.; 96: 1261-7.
  • Hustache G, Mollard A, Barnoud F. (1975) Culture illimitée d'une souche anergiée de Rosa glauca par la technique des suspensions cellulaires. C R Acad Sci Paris D.; 281: 1381-4.
  • Iwahara S, Maeyama T, Mishima T, Jikibara T, Takegawa K, Iwamoto H. (1992) Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1: IV. Isolation and identification of four novel oligosaccharide units derived from the acidic polysaccharide chain. J Biochem.; 112: 355-9.
  • Iwahara S, Suemori N, Ramli N, Takegawa K (1995) Isolation and identification of novel acidic oligosaccharides derived from glycoproteins of Fusarium sp. M7-1. Biosci Biotechnol Biochem.; 59: 1082-5.
  • Iwahara S, Suemori N, Takegawa K. (1996) Isolation and identification of a choline-linked mannobiose in the glycoproteins of Fusarium sp. M7-1. Biosci Biotechnol Biochem.; 60: 349-50.
  • Jikibara T, Takegawa K, Iwahara S. (1992a) Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1: I. Isolation and some properties of the glycoproteins. J Biochem.; 111: 225-9.
  • Jikibara T, Tada K, Takegawa K, Iwahara S. (1992b) Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1: II. The primary structures of the low molecular weight carbohydrate chains of the glycoproteins. J Biochem.; 111: 230-5.
  • Jikibara T, Takegawa K, Iwahara S. (1992c) Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1: III. The primary structures of the acidic polysaccharides of the glycoproteins. J Biochem.; 111: 236-43.
  • Kauss H, Jeblick W, Domard A. (1989) The degrees of polymerisation and N-acetylation of chitosan determine its ability to elicit callose formation in suspension cells and protoplasts of Catharanthus roseus. Plantae.; 178: 385-92.
  • Lee SW, Nazar RN, Powell DA, Robb J. (1992) Reduced PAL gene suppression in Verticillium-infected resistant tomatoes. Plant Mol Biol.; 18: 345-52.
  • Lesney MS. (1989) Growth responses and lignin production in cell suspensions of Pinus Elliotti 'elicited' by chitin, chitosan or mycelium of Cronartium quercum f. sp. fusiforme. Plant Cell Tissue Organ Cult.; 19: 23-31.
  • Ni W, Fahrendorf T, Ballance GM, Lamb CJ, Dixon RA. (1996) Stress responses in alfalfa (Medicago sativa L.). Transcriptional activation of phenylpropanoid pathway genes in elicitor-induced cell suspension cultures. Plant Mol Biol.; 30: 427-38.
  • Nita-Lazar M, Iwahara S, Takegawa K, Lienart Y. (2000) The active oxygen response of raspberry protoplasts to O-glycans of Fusarium L. sp. M7-1. J Plant Physiol.; 156: 306-11.
  • Nita-Lazar M, Chevolot L, Iwahara S, Takegawa K, Furmanek A, Lienart Y. (2002) High performance liquid chromatography and photodiode array detection of ferulic acid in Rubus protoplasts elicited by O-glycans from Fusarium sp. M7-1, Acta Biochim Polon.; 49: 1019-27.
  • Nurnberger T, Wirtz W, Nennstiel D, Hahlbrock K, Jabs T, Zimmermann S, Scheel D. (1997) Signal perception and intracellular signal transduction in plant pathogen defense. J Recept Signal Transduct Res.; 17: 127-36.
  • Prome JC. (1996) Signalling events elicited in plants by defined oligosaccharide structures. Curr Opin Struct Biol.; 6: 671-8.
  • Rodriguez-Galvez E, Mendgen K. (1995) Cell-wall synthesis in cotton roots after infection with Fusarium oxysporum. The deposition of callose, arabinogalactans, xyloglucans, and pectic components into walls, wall appositions, cell plates and plasmodesmata. Planta.; 197: 535-45.
  • Rouhier P, Kopp M, Begot V, Bruneteau M, Fritig B. (1995) Structural features of fungal beta-D-glucans for the efficient inhibition of the initiation of virus infection on Nicotiana tabacum. Phytochemistry.; 39: 57-62.
  • Shashkov AS, Nifantev NE, Amochaeva VY, Kochetkov NK. (1993) 1H and 13C NMR data for 2-O-, 3-O- and 2,3-di-O-glycosylated methyl alpha- and beta-D-glucopyranoses and beta-D-galactopyranosides. Magn Res Chem.; 31: 599-613.
  • Shaw NM, Bolwell GP, Smith C. (1990) Wound-induced phenylalanine ammonia-lyase in potato (Solanum tuberosum) tuber discs. Significance of glycosylation and immunolocalization of enzyme subunits. Biochem J.; 267: 163-70.
  • Zucker M. (1965) Induction of phenylalanine deaminase by light and its relation to chlorogenic acid synthesis in potato tuber tissue. Plant Physiol.; 40: 779-84.
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.