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In this study we have investigated the impact of differentiation of neuronal cells on their sensitivity to microbial toxins. We used the human neural crest-derived tumor cell line Paju, which can be induced to differentiation in vitro by treatment with phorbol 12-myristate 13-acetate. Addition of the highly toxic potassium ionophores cereulide (4.5 and 9.0 ng/ml) or valinomycin (20 ng/ml), to cultures of undifferentiated Paju cells caused collapse of the mitochondrial membrane potential - measured with the fluorescent probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetrabenzimidazole carbocyanine iodide (JC-1) followed by detachment of the cells and their apoptotic death. After induced differentiation of the Paju cells, their mitochondria retained the membrane potential upon exposure to the toxins and the cells displayed increased resistance to apoptosis as compared with undifferentiated cells. This effect may be caused by an elevated expression of the anti-apoptotic protein Bcl-2 and of the neuroprotective factor, stanniocalcin, in differentiated cells.
Journal
Year
Volume
Issue
Pages
539-544
Physical description
Dates
published
2004
received
2004-04-19
accepted
2004-04-23
Contributors
author
- Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, RU-142290 Pushchino, Moscow region, Russia
author
- Department of Applied Chemistry and Microbiology, Viikki Biocenter 1, University of Helsinki, Helsinki, Finland
author
- Department of Applied Chemistry and Microbiology, Viikki Biocenter 1, University of Helsinki, Helsinki, Finland
author
- Department of Applied Chemistry and Microbiology, Viikki Biocenter 1, University of Helsinki, Helsinki, Finland
author
- Department of Pathology, Haartman Institute, University of Helsinki, Helsinki, Finland
author
- Department of Pathology, Haartman Institute, University of Helsinki, Helsinki, Finland
author
- HusLab HUCH, Helsinki, Finland
References
- Andersson MA, Mikkola R, Kroppenstedt F, Rainey J, Reijula E, Hintikka E-L, Salkinoja-Salonen M. (1998a) Appl Environ Microbiol.; 64: 4767-73.
- Andersson MA, Mikkola R, Helin J, Andersson MC, Salkinoja-Salonen M. (1998b) Appl Environ Microbiol.; 64: 1338-43.
- Cai J, Yang J, Jones DP. (1998) Biochim Biophys Acta.; 1366: 139-49.
- Duax WL, Griffin JF, Langs DA, Smith GD, Groshulski P, Pletnev V, Ivanov V. (1996) Biopolymers.; 40: 141-55.
- Garlid KD, Paucek P. (2003) Biochim. Biophys. Acta.; 1606: 23-41.
- Gottlieb RA. (2000) FEBS Lett.; 482: 6-12.
- Inai Y, Yabuki M, Akiyama J, Yasuda K. (1997) Cell Struct Funct.; 22: 555-63.
- Krick S, Platoshin O, Sweeney M, Kim H, Yuan JX. (2001) Am J Physiol Cell Physiol.; 280: C970-9.
- Jääskeläinen E, Teplova V, Andersson MA, Andersson LC, Tammela P, Andersson MC, Pirhonen TI, Saris N-E, Vuorela P, Salkinoja-Salonen M. (2003) Toxicol In Vitro.; 17: 737-44.
- Lemasters JJ, Qian T, He L, Kim J-S, Elmore SP, Cascio WE, Brenner DA. (2002) Antioxid Redox Signal.; 4: 769-81.
- Li R, Mallahk RS. (2000) Neurosci Lett.; 294: 147-50.
- McCudden CR, James KA, Hasilo C, Wagner GF. (2002) J Biol Chem.; 277: 45249-58.
- Mikkola R, Saris N-E, Grigoriev PA, Andersson MA, Salkinoja-Salonen M. (1999) Eur J Biochem.; 263: 112-7.
- Murphy RC, Schneider E, Kinnally KW. (2001) FEBS Lett.; 497: 73-6.
- Paananen A, Mikkola R, Sareneva T, Matikainen S, Andersson M, Julkunen I, Salkinoja-Salonen M, Timonen T. (2000) Proc Natl Acad Sci USA.; 93: 4504-8.
- Paananen A, Mikkola R, Sareneva T, Matikainen S, Hess M, Andersson M, Julkunen I, Salkinoja-Salonen M, Timonen T. (2002) Clin Exp Immunol.; 129: 420-8.
- Penning LC, Denecker G, Vercammen D, Declercq W, Snipper RG, Vandenabeele P. (2000) Cytokine.; 12: 747-50.
- Reers M, Smiley ST, Mottola-Hartshorn C, Chen A, Lin M, Chen LB. (1995) Methods Enzymol.; 260: 406-17.
- Shimizu S, Eguchi Y, Kamiike W, Funahashi Y, Mignov A, Lacronique V, Matsuda H, Tsujimoto Y. (1998) Proc Natl Acad Sci USA.; 95: 1455-9.
- Shinohara Y, Almofti MR, Yamamoto T, Ishida T, Kita F, Kanzaki H, Ohnishi M, Yamashita K, Shimizu S, Terada H. (2002) Eur J Biochem.; 269: 5224-30.
- Yeh CJ, His BL, Faulk WP. (1981) J Immunol Methods.; 43: 269-75.
- Zhang KZ, Westberg JA, Hölttä E, Andersson LC. (1996) Proc Natl Acad Sci USA.; 93: 4504-8.
- Zhang KZ, Westberg JA, Paetau A, Bogyslawsky K, Lindsberg P, Erlander M, Guo H, Su J, Olsen HS, Andersson LC. (1998) Am J Pathol.; 153: 439-45.
- Zhang KZ, Lindsberg PJ, Tatlisumak T, Kaste M, Olsen HS, Andersson LC. (2000) Proc Natl Acad Sci USA.; 97: 3637-42.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv51i2p539kz