The contribution of uncoupling protein and ATP synthase to state 3 respiration in Acanthamoeba castellanii mitochondria.

• Authors: Wiesława Jarmuszkiewicz , Małgorzata Czarna , Claudine Sluse-Goffart , Francis E Sluse
• Languages of publication: EN

Abstracts

EN

Mitochondria of the amoeba Acanthamoeba castellanii possess a free fatty acid-activated uncoupling protein (AcUCP) that mediates proton re-uptake driven by the mitochondrial proton electrochemical gradient. We show that AcUCP activity diverts energy from ATP synthesis during state 3 mitochondrial respiration in a fatty acid-dependent way. The efficiency of AcUCP in mitochondrial uncoupling increases when the state 3 respiratory rate decreases as the AcUCP contribution is constant at a given linoleic acid concentration while the ATP synthase contribution decreases with respiratory rate. Respiration sustained by this energy-dissipating process remains constant at a given linoleic acid concentration until more than 60% inhibition of state 3 respiration by n-butyl malonate is achieved. The present study supports the validity of the ADP/O method to determine the actual contributions of AcUCP (activated with various linoleic acid concentrations) and ATP synthase in state 3 respiration of A. castellanii mitochondria fully depleted of free fatty acid-activated and describes how the two contributions vary when the rate of succinate dehydrogenase is decreased by succinate uptake limitation.

Keywords

EN

Acanthamoeba castellanii; uncoupling protein; energy-dissipating; mitochondria

• Journal: Acta Biochimica Polonica
• Year: 2004
• Volume: 51
• Issue: 2
• Pages: 533-538

Dates

published

2004

received

2004-04-30

accepted

2004-05-07

Contributors

author

Wiesława Jarmuszkiewicz

• Laboratory of Bioenergetics, Adam Mickiewicz University, Poznań, Poland

author

Małgorzata Czarna

• Laboratory of Bioenergetics, Adam Mickiewicz University, Poznań, Poland

author

Claudine Sluse-Goffart

• Laboratory of Bioenergetics, Department of Life Sciences, University of Liěge, Sart Tilman, Liěge, Belgium

author

Francis E Sluse

• Laboratory of Bioenergetics, Department of Life Sciences, University of Liěge, Sart Tilman, Liěge, Belgium

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