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2004 | 51 | 1 | 51-56
Article title

Tryptic digestion of peptides corresponding to modified fragments of human growth hormone-releasing hormone.

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EN
Abstracts
EN
The objective of this study was to examine the degradation of short peptides corresponding to modified fragments of human growth hormone-releasing hormone by trypsin. Six analogues of pentapeptide 9-13 of human growth hormone-releasing hormone containing homoarginine, ornithine, glutamic acid, glycine, leucine or phenylalanine residue in position 11, two analogues of hexapeptide 8-13 of human growth hormone-releasing hormone and two analogues of heptapeptide 7-13 of human growth hormone-releasing hormone containing homoarginine or glycine residue in position 11 were obtained. The peptides were subjected to digestion by trypsin and the course of reaction was monitored using HPLC. It was found that the rate of hydrolysis of the Lys12-Val13 peptide bond depends on the amino-acid residue preceding Lys12. The extension of the peptide chain towards the N-terminus by introduction of consecutive amino-acid residues corresponding to the human growth hormone-releasing hormone sequence accelerates the hydrolysis process. These results may be of assistance in designing new analogues of human growth hormone-releasing hormone, more resistant to the activity of proteolytic enzymes.
Publisher

Year
Volume
51
Issue
1
Pages
51-56
Physical description
Dates
published
2004
received
2003-10-31
revised
2004-02-04
accepted
2004-02-08
Contributors
author
  • Laboratory of Peptides, Faculty of Chemistry, Warsaw University, L. Pasteura 1, 02-093 Warszawa, Poland
  • Laboratory of Peptides, Faculty of Chemistry, Warsaw University, L. Pasteura 1, 02-093 Warszawa, Poland
author
  • Laboratory of Peptides, Faculty of Chemistry, Warsaw University, L. Pasteura 1, 02-093 Warszawa, Poland
References
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  • Frohman LA, Downs TR, Heimer EP, Felix AM. (1989) Dipeptidylpeptidase IV and trypsin-like enzymatic degradation of human growth hormone-releasing hormone in plasma. J Clin Invest.; 83: 1533-40.
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  • Wehrenberg WB, Ling N. (1983) In vivo biological potency of rat and human growth hormone-releasing factor and fragments of human growth hormone-releasing factor. Biochem Biophys Res Commun.; 115: 525-30.
  • Witkowska E, Orłowska A, Sagan B, Smoluch M, Izdebski J. (2001) Tryptic hydrolysis of hGH-RH(1-29)-NH2 analogues containing Lys or Orn in position 12 and 21. J Pept Sci.; 7: 166-72.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv51i1p51kz
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