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2004 | 51 | 1 | 219-222
Article title

Cadmium-induced changes in antioxidant enzymes in suspension culture of soybean cells.

Content
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EN
Abstracts
EN
Cadmium (Cd), similarly to other heavy metals, inhibits plant growth. We have recently showed that Cd2+ either stimulates (1-4 μM) or inhibits (ł 6 μM) growth of soybean (Glycine max L.) cells in suspension culture (Sobkowiak & Deckert, 2003, Plant Physiol Biochem. 41: 767-72). Here, soybean cell suspension cultures were treated with various concentrations of Cd2+ (1-10 μM) and the following enzymes were analyzed by native electrophoresis: superoxide dismutase (SOD), catalase (CAT), peroxidase (POX) and ascorbate peroxidase (APOX). We found a significant correlation between the cadmium-induced changes of soybean cell culture growth and the isoenzyme pattern of the antioxidant enzymes. The results suggest that inhibition of growth and modification of antioxidant defense reactions appear in soybean cells when Cd2+ concentration in culture medium increases only slightly, from 4 to 6 μM.
Publisher

Year
Volume
51
Issue
1
Pages
219-222
Physical description
Dates
published
2004
received
2003-08-07
revised
2003-12-05
accepted
2004-01-19
Contributors
  • Laboratory of Plant Ecophysiology, Faculty of Biology, Adam Mickiewicz University, Al. Niepodległości 14, 61-713 Poznań, Poland
  • Laboratory of Plant Ecophysiology, Faculty of Biology, Adam Mickiewicz University, Al. Niepodległości 14, 61-713 Poznań, Poland
  • Laboratory of Plant Ecophysiology, Faculty of Biology, Adam Mickiewicz University, Al. Niepodległości 14, 61-713 Poznań, Poland
  • Laboratory of Plant Ecophysiology, Faculty of Biology, Adam Mickiewicz University, Al. Niepodległości 14, 61-713 Poznań, Poland
References
  • Beauchamp Ch, Fridovich I. (1971) Anal Biochem.; 44: 276-87.
  • Bradford M. (1976) Anal Biochem.; 72: 248-54.
  • Chaoui A, Mazhoudi S, Ghorbal MH, El Ferjani E. (1997) Plant Sci.; 127: 139-47.
  • Davis BJ. (1964) Ann NY Acad Sci.; 121: 404-27.
  • Gallego SM, Benavides MP, Tomaro ML. (1996) Plant Sci.; 121: 151-9.
  • Halliwell B, Gutterdge JMC. (1989) In Free Radicals in Biology and Medicine. Halliwell B, Gutterdge JMC, eds, pp 86-123. Clarendon Press, Oxford.
  • Mittler R, Zilinskas BA. (1993) Anal Biochem.; 212: 540-6.
  • Olmos E, Martinez-Solano JR, Piqueras A, Hellin E. (2003) J Exp Bot.; 54: 291-301.
  • Ros Barceló A. (1987) Ann Biol.; 14: 33-8.
  • Rucińska R, Waplak S, Gwóźdź EA. (1999) Plant Physiol Biochem.; 37: 187-94.
  • Sandalio LM, Dalurzo HC, Gomez M, Romero-Puertas MC, del Rio LA. (2001) J Exp Bot.; 52: 2115-26.
  • Sanita di Toppi L, Gabbrielli R. (1999) Environ Exp Bot.; 41: 105-30.
  • Shaw BP. (1995) Biol Plant.; 37: 587-96.
  • Sobkowiak R, Deckert J. (2003) Plant Physiol Biochem.; 41: 767-72.
  • Szuster-Ciesielska A, Stachura A, Słotwińska M, Kamińska T, Śnieżko R, Paduch R, Abramczyk D, Filar J, Kandefer-Szerszeń M. (2000) Toxicology.; 145: 159-71.
  • Vitoria AP, Lea PJ, Azevedo RA. (2001) Phytochemistry.; 57: 701-10.
  • Woodbury W, Spencer AK, Stahmann MA. (1971) Anal Biochem.; 44: 301-5.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv51i1p219kz
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