Cadmium-induced changes in antioxidant enzymes in suspension culture of soybean cells.

• Authors: Robert Sobkowiak , Katarzyna Rymer , Renata Rucińska , Joanna Deckert
• Languages of publication: EN

Abstracts

EN

Cadmium (Cd), similarly to other heavy metals, inhibits plant growth. We have recently showed that Cd2+ either stimulates (1-4 μM) or inhibits (ł 6 μM) growth of soybean (Glycine max L.) cells in suspension culture (Sobkowiak & Deckert, 2003, Plant Physiol Biochem. 41: 767-72). Here, soybean cell suspension cultures were treated with various concentrations of Cd2+ (1-10 μM) and the following enzymes were analyzed by native electrophoresis: superoxide dismutase (SOD), catalase (CAT), peroxidase (POX) and ascorbate peroxidase (APOX). We found a significant correlation between the cadmium-induced changes of soybean cell culture growth and the isoenzyme pattern of the antioxidant enzymes. The results suggest that inhibition of growth and modification of antioxidant defense reactions appear in soybean cells when Cd2+ concentration in culture medium increases only slightly, from 4 to 6 μM.

Keywords

EN

ascorbate peroxidase; superoxide dismutase; peroxidase; soybean cell suspension culture; catalase; cadmium

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2004
• Volume: 51
• Issue: 1
• Pages: 219-222

Dates

published

2004

received

2003-08-07

revised

2003-12-05

accepted

2004-01-19

Contributors

author

Robert Sobkowiak

• Laboratory of Plant Ecophysiology, Faculty of Biology, Adam Mickiewicz University, Al. Niepodległości 14, 61-713 Poznań, Poland

author

Katarzyna Rymer

• Laboratory of Plant Ecophysiology, Faculty of Biology, Adam Mickiewicz University, Al. Niepodległości 14, 61-713 Poznań, Poland

author

Renata Rucińska

• Laboratory of Plant Ecophysiology, Faculty of Biology, Adam Mickiewicz University, Al. Niepodległości 14, 61-713 Poznań, Poland

author

Joanna Deckert

• Laboratory of Plant Ecophysiology, Faculty of Biology, Adam Mickiewicz University, Al. Niepodległości 14, 61-713 Poznań, Poland

References

• Beauchamp Ch, Fridovich I. (1971) Anal Biochem.; 44: 276-87.
• Bradford M. (1976) Anal Biochem.; 72: 248-54.
• Chaoui A, Mazhoudi S, Ghorbal MH, El Ferjani E. (1997) Plant Sci.; 127: 139-47.
• Davis BJ. (1964) Ann NY Acad Sci.; 121: 404-27.
• Gallego SM, Benavides MP, Tomaro ML. (1996) Plant Sci.; 121: 151-9.
• Halliwell B, Gutterdge JMC. (1989) In Free Radicals in Biology and Medicine. Halliwell B, Gutterdge JMC, eds, pp 86-123. Clarendon Press, Oxford.
• Mittler R, Zilinskas BA. (1993) Anal Biochem.; 212: 540-6.
• Olmos E, Martinez-Solano JR, Piqueras A, Hellin E. (2003) J Exp Bot.; 54: 291-301.
• Ros Barceló A. (1987) Ann Biol.; 14: 33-8.
• Rucińska R, Waplak S, Gwóźdź EA. (1999) Plant Physiol Biochem.; 37: 187-94.
• Sandalio LM, Dalurzo HC, Gomez M, Romero-Puertas MC, del Rio LA. (2001) J Exp Bot.; 52: 2115-26.
• Sanita di Toppi L, Gabbrielli R. (1999) Environ Exp Bot.; 41: 105-30.
• Shaw BP. (1995) Biol Plant.; 37: 587-96.
• Sobkowiak R, Deckert J. (2003) Plant Physiol Biochem.; 41: 767-72.
• Szuster-Ciesielska A, Stachura A, Słotwińska M, Kamińska T, Śnieżko R, Paduch R, Abramczyk D, Filar J, Kandefer-Szerszeń M. (2000) Toxicology.; 145: 159-71.
• Vitoria AP, Lea PJ, Azevedo RA. (2001) Phytochemistry.; 57: 701-10.
• Woodbury W, Spencer AK, Stahmann MA. (1971) Anal Biochem.; 44: 301-5.