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2004 | 51 | 1 | 189-197
Article title

Insertion of GPI-anchored alkaline phosphatase into supported membranes: a combined AFM and fluorescence microscopy study.

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EN
Abstracts
EN
A new method based on combined atomic force microscopy (AFM) and fluorescence microscopy observations, is proposed to visualize the insertion of glycosylphosphatidyl inositol (GPI) anchored alkaline phosphatase from buffer solutions into supported phospholipid bilayers. The technique involves the use of 27 nm diameter fluorescent latex beads covalently coupled to the amine groups of proteins. Fluorescence microscopy allows the estimation of the relative protein coverage into the membrane and also introduces a height amplification for the detection of protein/bead complexes with the AFM. The coupling of the beads with the amine groups is not specific; this new and simple approach opens up new ways to investigate proteins into supported membrane systems.
Publisher

Year
Volume
51
Issue
1
Pages
189-197
Physical description
Dates
published
2004
received
2003-12-19
revised
2004-01-07
accepted
2004-02-06
Contributors
  • Laboratoire de Physique de la Matière Condensée et Nanostructures, Université Claude Bernard Lyon-1, 69622 Villeurbanne, France
  • Laboratoire de Physique de la Matière Condensée et Nanostructures, Université Claude Bernard Lyon-1, 69622 Villeurbanne, France
  • Laboratoire de Physique, ENS Lyon, 69364 Lyon cedex 07, Lyon, France
  • Laboratoire Pollution et Traitement des Eaux, Université Mentouri-Constantine, 25000 Constantine, Algeria
  • Laboratoire de Physique de la Matière Condensée et Nanostructures, Université Claude Bernard Lyon-1, 69622 Villeurbanne, France
author
  • Laboratoire de Physique de la Matière Condensée et Nanostructures, Université Claude Bernard Lyon-1, 69622 Villeurbanne, France
References
  • Angrand M, Briolay A, Ronzon F, Roux B. (1997) Detergent-mediated reconstitution of a glycosylphosphatidylinositol-protein into liposomes. Eur J Biochem.; 250: 168-76.
  • Czajkowsky DM, Iwamoto H, Cover TL, Shao Z. (1999) The vacuolating toxin from Helicobacter pylori forms hexameric pores in lipid bilayers at low pH. Proc Nat Acad Sci USA.; 96: 2001-6.
  • Dufrêne YF, Lee GU. (2000) Direct characterization of the physicochemical properties of fungal spores using functionalized AFM probes. Biochim Biophys Acta.; 1509: 14-41.
  • Ferguson MA. (1999) The structure, biosynthesis and functions of glycosylphosphatidylinositol anchors, and the contributions of trypanosome research. J Cell Sci.; 112: 2799-809.
  • Kaasgaard T, Mouritsen OG, Jorgensen K. (2002) Lipid domain formation and ligand-receptor distribution in lipid bilayer membranes investigated by atomic force microscopy. FEBS Lett.; 515: 29-34.
  • Le Du MH, Stigbrand T, Taussig MJ, Menez A, Stura EA. (2001) Crystal structure of alkaline phosphatase from human placenta at 1.8 Å resolution. Implication for a substrate specificity. J Biol Chem.; 276: 9158-65.
  • Lehto MT, Sharom FJ. (2002) Proximity of the protein moiety of a GPI-anchored protein to the membrane surface: a FRET study. Biochemistry.; 41: 8368-76.
  • Milhiet PE, Giocondi MC, Baghdadi O, Ronzon F, Roux B, LeGrimellec C. (2002a) Spontaneous insertion and partitioning of alkaline phosphatase into model lipid rafts. EMBO Rep.; 3: 485-90.
  • Milhiet PE, Giocondi MC, Baghdadi O, Ronzon F, LeGrimellec C, Roux B. (2002b) AFM characterization of model rafts in supported bilayers. Single Mol.; 3: 136-41.
  • Morandat S, Bortolato M, Roux B (2002) Cholesterol-dependent insertion of glycosylphosphatidylinositol-anchored enzyme. Biochim Biophys Acta.; 1564: 473-8.
  • Mou J, Czajkowsky DM, Shao Z. (1996) Gramicidin A aggregation in supported gel state phosphatidylcholine bilayers. Biochemistry.; 35: 3222-26.
  • Nosjean O, Briolay A, Roux B. (1997) Mammalian GPI proteins: sorting, membrane residence and functions. Biochim Biophys Acta.; 1331: 153-86.
  • Nosjean O, Roux B. (1999) Ectoplasmic insertion of a glycosylphosphatidylinositol-anchored protein in glycosphingolipid- and cholesterol-containing phosphatidylcholine vesicles. Eur J Biochem.; 263: 865-70.
  • Perrin A, Theretz A, Lanet V, Vialle S, Mandrand B. (1999) Immunomagnetic concentration of antigens and detection based on a scanning force microscopic immunoassay. J Immunol Methods.; 224: 77-87.
  • Putman CAJ, van Leeuwen AM, de Grooth BG, Radosevic K, van der Werf K, van Hulst NF, Greve J. (1993) Atomic force microscopy combined with confocal laser scanning microscopy: A new look at cells. Bioimaging.; 1: 63-70.
  • Puu G, Artursson E, Gustafson I, Lundstrom M, Jass J. (2000) Distribution and stability of membrane proteins in lipid membranes on solid supports. Biosens Bioelectron.; 15: 31-41.
  • Read SM, Northcote DH. (1981) Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein. Anal Biochem.; 116: 53-64.
  • Rieu J-P, Ronzon F, Roux B. (2002) Adsorption and aggregation of glycosylphosphatidyl inositol (GPI) anchored alkaline phosphatase on methylated glass surfaces studied by tapping mode atomic force microscopy. Thin Solid Films.; 406: 241-9.
  • Rinia HA, de Kruijff B. (2001) Imaging domains in model membranes with atomic force microscopy. FEBS Lett.; 504: 194-9.
  • Ronzon F, Desbat B, Buffeteau T, Mingotaud C, Chauvet J-P, Roux B. (2002) Structure and orientation of a glycosylphosphatidyl inositol anchored protein at the air/water interface. J Phys Chem B.; 106: 3307-15.
  • Sackmann E. (1996) Supported membranes: scientific and practical applications. Science.; 271: 43-8.
  • Saslowsky DE, Lawrence J, Ren X, Brown DA, Henderson RM, Edwardson JM. (2002) Placental alkaline phosphatase is efficiently targeted to rafts in supported lipid bilayers.J Biol Chem.; 277: 26966-70.
  • Schabert F, Knapp H, Karrasch S, Häring R, Engel A. (1994) Confocal scanning laser scanning probe hybrid microscope for biological applications. Ultramicroscopy.; 53: 147-57.
  • Slade A, Luh J, Ho S, Yip CM. (2002) Single molecule imaging of supported planar lipid bilayer reconstituted human insulin receptors by in situ scanning probe microscopy.J Struct Biol.; 137: 283-91.
  • Werten PJ, Rémigy H-W, de Groot BL, Fotiadis D, Philippsen A, Stahlberg H, Grubmüller H, Engel A. (2002) Progress in the analysis of membrane protein structure and function. FEBS Lett.; 529: 65-72.
  • Yuan C, Johnston LJ. (2001) Atomic force microscopy studies of ganglioside GM1 domains in phosphatidylcholine and phosphatidylcholine/cholesterol bilayers. Biophys J.; 81: 1059-69.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv51i1p189kz
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