Effect of antisense peptide binding on the dimerization of human cystatin C - gel electrophoresis and molecular modeling studies.

• Authors: Krystyna Stachowiak , Sylwia Rodziewicz-Motowidło , Renata Sosnowska , Franciszek Kasprzykowski , Leszek Łankiewicz , Anders Grubb , Zbigniew Grzonka
• Languages of publication: EN

Abstracts

EN

Human cystatin C (HCC) shows a tendency to dimerize. This process is particularly easy in the case of the L68Q HCC mutant and might lead to formation of amyloid deposits in brain arteries of young adults. Our purpose was to find ligands of monomeric HCC that can prevent its dimerization. Eleven antisense peptide ligands of monomeric HCC were designed and synthesized. The influence of these ligands on HCC dimerization was studied using gel electrophoresis and molecular modeling methods. The results suggest that all the designed peptides interact with monomeric HCC facilitating its dimerization rather than preventing it.

Keywords

EN

dimerization; antisense peptides; human cystatin C; molecular dynamics

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2004
• Volume: 51
• Issue: 1
• Pages: 153-160

Dates

published

2004

received

2003-10-31

revised

2004-03-02

accepted

2004-03-09

Contributors

author

Krystyna Stachowiak

• Faculty of Chemistry, University of Gdańsk, Gdańsk, Poland

author

Sylwia Rodziewicz-Motowidło

• Faculty of Chemistry, University of Gdańsk, Gdańsk, Poland

author

Renata Sosnowska

• Faculty of Chemistry, University of Gdańsk, Gdańsk, Poland

author

Franciszek Kasprzykowski

• Faculty of Chemistry, University of Gdańsk, Gdańsk, Poland

author

Leszek Łankiewicz

• Faculty of Chemistry, University of Gdańsk, Gdańsk, Poland

author

Anders Grubb

• Department of Clinical Chemistry, University Hospital, Lund, Sweden

author

Zbigniew Grzonka

• Faculty of Chemistry, University of Gdańsk, Gdańsk, Poland

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