Interaction of class A G protein-coupled receptors with G protein.

• Authors: Rafał Ślusarz , Jerzy Ciarkowski
• Languages of publication: EN

Abstracts

EN

A model for interaction of class A G protein-coupled receptor with the G protein Gα subunit is proposed using the rhodopsin-transducin (RD/Gt) prototype. The model combines the resolved interactions/distances, essential in the active RD*/Gt system, with the structure of Gtα C-terminal peptide bound to RD* while stabilizing it. Assuming the interactions involve conserved parts of the partners, the model specifies the conserved Helix 2 non-polar X- - -X, Helix 3 DRY and Helix 7/8 NP- -Y- - F RD* motifs interacting with the Gtα C-terminal peptide, in compliance with the structure of the latter. A concomitant role of Gtα and Gtγ>C-termini in stabilizing RD* could possibly be resolved assuming a receptor dimer as requisite for G protein activation.

Keywords

EN

GPCR class A; GPCR activation; G protein

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2004
• Volume: 51
• Issue: 1
• Pages: 129-136

Dates

published

2004

received

2003-10-31

revised

2004-02-01

accepted

2004-03-04

Contributors

author

Rafał Ślusarz

• Faculty of Chemistry, University of Gdansk, Gdańsk, Poland

author

Jerzy Ciarkowski

• Faculty of Chemistry, University of Gdansk, Gdańsk, Poland

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