Paul-Bunnell antigen and a possible mechanism of formation of heterophile antibodies in patients with infectious mononucleosis.

• Authors: Ewa Gołaszewska , Ewa Kurowska , Maria Duk , Jerzy Kościelak
• Languages of publication: EN

Abstracts

EN

Sera of patients with infectious mononucleosis contain heterophile anti-Paul- Bunnell (PB) antibodies to erythrocytes of numerous mammalian species. Evidence is presented that the corresponding antigen of bovine erythrocytes is not, as previously described, a single molecule, but a series of glycoproteins with glycans terminated with N-glycolylneuraminic acid (Neu5Gc). The latter compound should be an important part of the PB epitope because, in agreement with the results of others, we found that desialylation of the PB antigen abolishes almost completely its activity. We examined three different preparations of GM3 ganglioside for their capacity to bind anti-PB and found that only GM3 from horse erythrocytes containing Neu5Gc exhibited a low although ELISA measurable PB activity. The other two GM3 preparations, from bovine milk and dog erythrocytes, containing N-acetylneuraminic acid (Neu5Ac) bound little if any anti-PB antibodies. This finding confirms a previous report that human erythrocyte Neu5Ac containing sialoglycoprotein with similar O-linked glycans as the PB-antigen of bovine erythrocytes exhibits only very low PB activity (Patarca & Fletcher, 1995, Crit Rev Oncogen., 6: 305). In conclusion, we present a hypothesis that anti-PB antibodies in patients with infectious mononucleosis are formed against infection-induced cell membrane glycoconjugates containing highly immunogenic Neu5Gc.

Keywords

EN

infectious mononucleosis; Paul-Bunnell antigen; Hanganutziu-Deicher antigen; N-glycolylneuraminic acid

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2003
• Volume: 50
• Issue: 4
• Pages: 1205-1211

Dates

published

2003

received

2003-08-18

revised

2003-11-25

accepted

2003-12-03

Contributors

author

Ewa Gołaszewska

• Department of Biochemistry, Institute of Hematology and Blood Transfusion, Warszawa, Poland

author

Ewa Kurowska

• Department of Biochemistry, Institute of Hematology and Blood Transfusion, Warszawa, Poland

author

Maria Duk

• Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wrocław, Poland

author

Jerzy Kościelak

• Department of Biochemistry, Institute of Hematology and Blood Transfusion, Warszawa, Poland

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