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2003 | 50 | 4 | 1165-1174
Article title

Photodynamic effects of two water soluble porphyrins evaluated on human malignant melanoma cells in vitro.

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EN
Abstracts
EN
Two water soluble porphyrins: meso-tetra-4-N-methylpyridyl-porphyrin iodide (P1) and 5,10-di-(4-acetamidophenyl)-15,20-di-(4-N-methylpyridyl) porphyrin (P2) were synthesised and evaluated in respect to their photochemical and photophysical properties as well as biological activity. Cytotoxic and phototoxic effects were evaluated in human malignant melanoma Me45 line using clonogenic assay, cytological study of micronuclei, apoptosis and necrosis frequency and inhibition of growth of megacolonies. Both porphyrins were characterised by high UV and low visible light absorptions. Dark toxicity measured on the basis of the clonogenic assay and inhibition of megacolony growth area indicated that P1 was non-toxic at concentrations up to 50 μg/ml (42.14 μM) and P2 at concentrations up to 20 μg/ml (16.86 μM). The photodynamic effect induced by red light above 630 nm indicated that both porphyrins were able to inhibit growth of melanoma megacolonies at non-toxic concentrations. Cytologic examination showed that the predominant mode of cell death was necrosis.
Year
Volume
50
Issue
4
Pages
1165-1174
Physical description
Dates
published
2003
received
2003-05-17
revised
2003-09-28
accepted
2003-11-14
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv50i4p1165kz
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