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2003 | 50 | 4 | 1155-1163
Article title

Application of electrophoretic methods for detection of protein-porphyrin complexes.

Content
Title variants
Languages of publication
EN
Abstracts
EN
Simple methods for detection and isolation of protein-porphyrin complexes were elaborated in our laboratory. They are based on the separation of protein-porphyrin complexes in native polyacrylamide gel and measurement of their fluorescence, with the use of two detection systems: the commercially available Gel DocTM 2000 system, and a system specially designed for the purpose of these investigations, concerning protein-porphyrin interactions. The fluorescent complexes can be electro-transferred from the gel onto PVDF membrane, eluted and analyzed in order to identify the protein interacting with porphyrins.
Publisher

Year
Volume
50
Issue
4
Pages
1155-1163
Physical description
Dates
published
2003
received
2003-10-13
revised
2003-11-26
accepted
2003-12-09
Contributors
  • Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland
author
  • Department of Acousto-optics and Laser Physics, Institute of Experimental Physics, Faculty of Mathematics, Physics and Informatics, University of Gdańsk, Gdańsk, Poland
author
  • Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland
  • Department of Acousto-optics and Laser Physics, Institute of Experimental Physics, Faculty of Mathematics, Physics and Informatics, University of Gdańsk, Gdańsk, Poland
  • Institute of Optoelectronics, Military University of Technology, Warszawa, Poland
  • Institute of Optoelectronics, Military University of Technology, Warszawa, Poland
  • Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv50i4p1155kz
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