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2003 | 50 | 4 | 1064-1073
Article title

Expression level of Ubc9 protein in rat tissues.

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EN
Abstracts
EN
Ubc9 is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. In this report we describe a simple and efficient method for obtaining pure human recombinant Ubc9 protein. The purified Ubc9 retained its native structure and was fully active in an in vitro sumoylation assay with the promyelocytic leukaemia (PML) peptide as a substrate. In order to better understand the physiology of Ubc9 protein we examined its levels in several rat tissues. Immunoblot analyses performed on tissue extracts revealed quantitative and qualitative differences in the expression pattern of Ubc9. The Ubc9 protein was present at a high level in spleen and lung. Moderate level of Ubc9 was detected in kidney and liver. Low amount of Ubc9 was observed in brain, whereas the 18 kDa band of Ubc9 was barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the Ubc9 antibodies recognized a 38 kDa protein band. This band was not visible in extracts of other rat tissues. A comparison of the relative levels of Ubc9 mRNA and protein indicated that the overall expression level of Ubc9 was the highest in spleen and lung. In spleen, lung, kidney, brain, liver and heart there was a good correlation between the 18 kDa protein and Ubc9 mRNA levels. In skeletal muscle the Ubc9 mRNA level was unproportionally high comparing to the level of the 18 kDa protein. The presented data indicate that in the rat the expression of the Ubc9 protein appears to have some degree of tissue specificity.
Publisher

Year
Volume
50
Issue
4
Pages
1064-1073
Physical description
Dates
published
2003
received
2003-09-27
revised
2003-11-28
accepted
2003-12-09
Contributors
  • Department of Molecular Medicine, Medical University of Gdańsk, 80-211 Gdańsk, Poland
author
  • Department of Molecular Medicine, Medical University of Gdańsk, 80-211 Gdańsk, Poland
  • Department of Molecular Medicine, Medical University of Gdańsk, 80-211 Gdańsk, Poland
  • Department of Laboratory Medicine, Medical University of Gdańsk, 80-211 Gdańsk, Poland
  • Department of Molecular Medicine, Medical University of Gdańsk, 80-211 Gdańsk, Poland
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Document Type
Publication order reference
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YADDA identifier
bwmeta1.element.bwnjournal-article-abpv50i4p1064kz
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