YOR129c, a new element interacting with Cnm67p, a component of the spindle pole body of Saccharomyces cerevisiae.

• Authors: Monika Wysocka , Agnieszka Białkowska , Arkadiusz Miciałkiewicz , Anna Kurlandzka
• Languages of publication: EN

Abstracts

EN

The Saccharomyces cerevisiae spindle pole body (SPB) consists of numerous proteins forming the outer, inner and central plaques. The protein Cnm67 is an important component of the outer plaque. The C-terminus of this protein contains a determinant important for its SPB localization. We identified a protein encoded by YOR129c which interacts with this C-terminus in the two-hybrid system. YOR129c and CNM67 exhibit weak genetic interaction. The double deletion strain yor129cΔ cnm67Δ exhibits moderately increased resistance to 0.1 M LiCl and hygromycin B compared with the cnm67Δ single mutant. We propose that the YOR129c protein is an accessory factor associated with the cytoplasmic face of SPB and plays a role in cation homeostasis and/or multidrug resistance.

Keywords

EN

hygromycin B; spindle pole body; Cnm67; lithium tolerance; yeast

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2003
• Volume: 50
• Issue: 3
• Pages: 883-890

Dates

published

2003

received

2003-06-26

revised

2003-08-11

accepted

2003-08-19

Contributors

author

Monika Wysocka

• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, A. Pawińskiego 5A, 02-106 Warszawa, Poland

author

Agnieszka Białkowska

• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, A. Pawińskiego 5A, 02-106 Warszawa, Poland

author

Arkadiusz Miciałkiewicz

• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, A. Pawińskiego 5A, 02-106 Warszawa, Poland

author

Anna Kurlandzka

• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, A. Pawińskiego 5A, 02-106 Warszawa, Poland

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