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2003 | 50 | 3 | 883-890
Article title

YOR129c, a new element interacting with Cnm67p, a component of the spindle pole body of Saccharomyces cerevisiae.

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The Saccharomyces cerevisiae spindle pole body (SPB) consists of numerous proteins forming the outer, inner and central plaques. The protein Cnm67 is an important component of the outer plaque. The C-terminus of this protein contains a determinant important for its SPB localization. We identified a protein encoded by YOR129c which interacts with this C-terminus in the two-hybrid system. YOR129c and CNM67 exhibit weak genetic interaction. The double deletion strain yor129cΔ cnm67Δ exhibits moderately increased resistance to 0.1 M LiCl and hygromycin B compared with the cnm67Δ single mutant. We propose that the YOR129c protein is an accessory factor associated with the cytoplasmic face of SPB and plays a role in cation homeostasis and/or multidrug resistance.
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