Preferences help
enabled [disable] Abstract
Number of results
2003 | 50 | 3 | 865-873
Article title

The bovine tyrosine hydroxylase gene associates in vitro with the nuclear matrix by its first intron sequence*.;

Title variants
Languages of publication
Recently we have shown that in vitro binding of the proximal part of the human tyrosine hydroxylase gene to the nuclear matrix is correlated with its transcriptional activity. The strongest binding potential was predicted by computing for the first intron sequence (Lenartowski & Goc, 2002, Neurosci Lett.; 330 : 151-154). In this study a 16 kb fragment of the bovine genomic DNA containing the tyrosine hydroxylase gene was investigated for its affinity to the nuclear matrix. Only a 950 bp fragment encoding the distal part of the first intron, second exon and a few nucleotides of the second intron bound to the nuclear matrix. The binding was independent of the tissue-specific tyrosine hydroxylase gene activation. The fragment was subcloned and sequenced. Computer search pointed to one potential intronic matrix attachment region with two AP1-like sites embedded in the sequence. We conclude that even if the position of the matrix binding region is conserved among the tyrosine hydroxylase genes in mammals, its tissue specificity and/or function is not preserved or is achieved by different mechanisms.
Physical description
  • Albanese V, Biguet NF, Kiefer H, Bayard E, Mallet J, Meloni R. (2001) Quantitative effects on gene silencing by allelic variation at a tetranucleotide microsatellite. Hum Mol Genet.; 10: 1785-92.
  • Berezney R, Bucholtz LA. (1981) Dynamic association of replicating DNA fragments with the nuclear matrix of regenerating liver. Exp Cell Res.; 132: 1-13.
  • Berezney R, Coffey DS. (1977). Identification of a nuclear protein matrix. Biochem Biophys Res Commun.; 60: 1410-7.
  • Bidwell JP, van Wijnen AJ, Fey EG, Dworetzky S, Penman S, Stein JL, Lian JB, Stein GS. (1993) Osteocalcin gene promoter-binding factors are tissue-specific nuclear matrix components. Proc Natl Acad Sci U S A.; 90: 3162-6.
  • Bidwell JP, Fey EG, van Wijnen AJ, Penman S, Stein JL, Lian JB, Stein GS. (1994) Nuclear matrix proteins distinguish normal diploid osteoblasts from osteosarcoma cells. Cancer Res.; 54: 28-32.
  • Boulikas T. (1995) Chromatin domains and prediction of MAR sequences. Int Rev Cytol.; 162A: 279-388.
  • Cambi F, Fung B, Chikaraishi D. (1989) 5' flanking DNA sequences direct cell-specific expression of rat tyrosine hydroxylase. J Neurochem.; 53: 1656-9.
  • Ciejek EM, Tsai MJ, O'Malley BW. (1983) Actively transcribed genes are associated with the nuclear matrix. Nature.; 306: 607-9.
  • Cockerill PN, Garrard WT. (1986) Chromosomal loop anchorage sites appear to be evolutionarily conserved. FEBS Lett.; 204: 5-7.
  • Cohn MA, Hjelmso I, Wu LC, Guldberg P, Lukanidin EM, Tulchinsky EM. (2001) Characterization of Sp1, AP-1, CBF and KRC binding sites and minisatellite DNA as functional elements of the metastasis-associated mts1/S100A4 gene intronic enhancer. Nucleic Acids Res.; 29: 3335-46.
  • Coker 3rd GT, Vinnedge L, O'Malley KL. (1988) Characterization of rat and human tyrosine hydroxylase genes: functional expression of both promoters in neuronal and non-neuronal cell types. Biochem Biophys Res Commun.; 157: 1341-7.
  • D'Mello SR, Turzai LM, Gioio AE, Kaplan BB. (1989) Isolation and structural characterization of the bovine tyrosine hydroxylase gene. J Neurosci Res.; 23: 31-40.
  • Dworetzky SI, Fey EG, Penman S, Lian JB, Stein JL, Stein GS. (1990) Progressive changes in the protein composition of the nuclear matrix during rat osteoblast differentiation. Proc Natl Acad Sci U S A.; 87: 4605-9.
  • Fey EG, Penman S. (1988) Nuclear matrix proteins reflect cell type of origin in cultured human cells. Proc Natl Acad Sci U S A.; 85: 121-5.
  • Gandelman KY, Coker 3rd GT, Moffat M, O'Malley KL. (1990) Species and regional differences in the expression of cell-type specific elements at the human and rat tyrosine hydroxylase gene loci. J Neurochem.; 55: 2149-52.
  • Gasser SM, Laemmli UK. (1986) The organisation of chromatin loops: characterization of a scaffold attachment site. EMBO J.; 46: 521-30.
  • Gauss KA, Bunger PL, Quinn MT. (2002) AP-1 is essential for p67(phox) promoter activity. J Leukoc Biol.; 71: 163-72.
  • Getzenberg RH, Pienta KJ, Huang EY, Coffey DS. (1991) Identification of nuclear matrix proteins in the cancer and normal rat prostate. Cancer Res.; 51: 6514-20.
  • Greenstein RJ. (1988) Constitutive attachment of murine erythroleukemia cell histone-depleted DNA loops to nuclear scaffolding is found in the beta-major but not the alpha 1-globin gene. DNA.; 7: 601-7.
  • Iwata N, Kobayashi K, Sasaoka T, Hidaka H, Nagatsu T. (1992) Structure of the mouse tyrosine hydroxylase gene. Biochem Biophys Res Commun.; 182: 348-54.
  • Joaquin M, Salvado C, Fernandez de Mattos S, Tauler A. (2002) An intronic AP-1 sequence mediates the transcriptional activation of the F-type 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase by serum. Biochim Biophys Acta.; 1574: 131-6.
  • Katai H, Stephenson JD, Simkevich CP, Thompson JP, Raghow R. (1992) An AP-1-like motif in the first intron of human Pro alfa 1(I) collagen gene is a critical determinant of its transcriptional activity. Mol Cell Biochem.; 118: 119-29.
  • Käs E, Chasin LA. (1987) Anchorage of the Chinese hamster dihydrofolate reductase gene to the nuclear scaffold occurs in an intragenic region. J Mol Biol.; 198: 677-92.
  • Kilbourne EJ, Osaka H, Sabban EL. (1991) Hypomethylation of the rat tyrosine hydroxylase gene correlates with its expression in several cell types. Brain Res Dev Brain Res.; 58: 143-6.
  • Korosec T, Gerner C, Sauermann G. (1997) Common nuclear matrix proteins in rat tissues. Electrophoresis.; 18: 2109-15.
  • Kumer SC, Vrana KE. (1996) Intricate regulation of tyrosine hydroxylase activity and gene expression. J Neurochem.; 67: 443-62.
  • Lenartowski R, Goc A. (2002) Tissue-specific association of the human tyrosine hydroxylase gene with the nuclear matrix. Neurosci Lett.; 330: 151-4.
  • Lenartowski R, Pluskota W, Wojciechowski W, Goc A. (1997) Cell Mol Biol Lett.; 2: 232.
  • Lu A, Gupta A, Li C, Ahlborn TE, Ma Y, Shi EY, Liu J. (2001) Molecular mechanisms for aberrant expression of the human breast cancer specific gene 1 in breast cancer cells: control of transcription by DNA methylation and intronic sequences. Oncogene.; 20: 5173-85.
  • Meloni R, Albanese V, Ravassard P, Treilhou F, Mallet J. (1998) A tetranucleotide polymorphic microsatellite, located in the first intron of the tyrosine hydroxylase gene, acts as a transcription regulatory element in vitro. Hum Mol Genet.; 7: 423-8.
  • Mirkovitch J, Mirault ME, Laemmli UK. (1984) Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold. Cell.; 39: 223-32.
  • Partin AW, Getzenberg RH, CarMichael MJ, Vindivich D, Yoo J, Epstein JI, Coffey DS. (1993) Nuclear matrix protein patterns in human benign prostatic hyperplasia and prostate cancer. Cancer Res.; 53: 744-6.
  • Robinson SI, Small D, Idzerda R, McKnight GS, Vogelstein B. (1983) The association of transcriptionally active genes with the nuclear matrix of the chicken oviduct. Nucleic Acids Res.; 11: 5113-30.
  • Schimmel JJ, Crews L, Roffler-Tarlov S, Chikaraishi DM. (1999) 4.5 kb of the rat tyrosine hydroxylase 5' flanking sequence directs tissue specific expression during development and contains consensus sites for multiple transcription factors. Brain Res Mol Brain Res.; 74: 1-14.
  • Seshasayee D, Geiger JN, Gaines P, Wojchowski DM. (2000) Intron 1 elements promote erythroid-specific GATA-1 gene expression. J Biol Chem.; 275: 22969-77.
  • van Wijnen AJ, Bidwell JP, Fey EG, Penman S, Lian JB, Stein JL, Stein GS. (1993) Nuclear matrix association of multiple sequence-specific DNA binding activities related to SP-1, ATF, CCAAT, C/EBP, OCT-1, and AP-1. Biochemistry.; 32: 8397-402.
  • Wong SC, Moffat MA, Coker GT, Merlie JP, O'Malley KL. (1995) The 3' flanking region of the human tyrosine hydroxylase gene directs reporter gene expression in peripheral neuroendocrine tissues. J Neurochem.; 65: 23-31.
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.