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2003 | 50 | 3 | 799-806
Article title

X-band and S-band EPR detection of nitric oxide in murine endotoxaemia using spin trapping by ferro-di(N-(dithiocarboxy)sarcosine).

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Ammonium salt of N-(dithiocarboxy)sarcosine (DTCS) chelated to ferrous salt was tested as an NO-metric spin trap at room temperature for ex vivo measurement of g·NO production in murine endotoxaemia. In a chemically defined in vitro model system EPR triplet signals of NO-Fe(DTCS)g2 were observed for as long as 3 hours, only if samples were reduced with sodium dithionite. This procedure was not necessary for the ex vivo detection of ·NO in endotoxaemic liver homogenates at X-band or in the whole intact organs at S-band, whereas only a weak signal was observed in endotoxaemic lung. These results suggest that in endotoxaemia not only high level of ·NO, but also the redox properties of liver and lung might determine the formation of complexes of ·NO with a spin trap. Nevertheless, both S- and X-band EPR spectroscopy is suitable for ·NO-metry at room temperature using Fe(DTCS)2 as the spin trapping agent. In particular, S-band EPR spectroscopy enables the detection of ·NO production in a whole organ, such as murine liver.
Physical description
  • Department of Biophysics, Faculty of Biotechnology, Jagiellonian University, Kraków, Poland
  • Department of Biophysics, Faculty of Biotechnology, Jagiellonian University, Kraków, Poland
  • Department of Pharmacology, Collegium Medicum of the Jagiellonian University, Kraków, Poland
  • The Center for Low Frequency EPR Imaging for In Vivo Physiology, The University of Chicago, IL, U.S.A., and University of Maryland, Baltimore, MD, U.S.A.
  • Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, The Medical Biotechnology Center, University of Maryland Biotechnology Institutes, Baltimore, MD, U.S.A.
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