Desorption/ionization on silicon for small molecules: a promising alternative to MALDI TOF.

• Authors: Agnieszka Kraj , Tomasz Dylag , Anna Gorecka-Drzazga , Sylwester Bargiel , Jan Dziuban , Jerzy Silberring
• Languages of publication: EN

Abstracts

EN

A method has been developed for laser desorption/ionization of catecholamines from porous silicon. This methodology is particularly attractive for analysis of small molecules. MALDI TOF mass spectrometry, although a very sensitive technique, utilizes matrices that need to be mixed with the sample prior to their analysis. Each matrix produces its own background, particularly in the low-molecular mass region. Therefore, detection and identification of molecules below 400 Da can be difficult. Desorption/ionization of samples deposited on porous silicon does not require addition of a matrix, thus, spectra in the low-molecular mass region can be clearly readable. Here, we describe a method for the analysis of catecholamines. While MALDI TOF is superior for proteomics/peptidomics, desorption/ionization from porous silicon can extend the operating range of a mass spectrometer for studies on metabolomics (small organic molecules and their metabolites, such as chemical neurotransmitters, prostaglandins, steroids, etc.).

Keywords

EN

proteomics; porous silicon; catecholamines; peptides; DIOS; mass spectrometry; desorption/ionization; MALDI TOF

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2003
• Volume: 50
• Issue: 3
• Pages: 783-787

Dates

published

2003

received

2003-05-30

revised

2003-08-23

accepted

2003-09-09

Contributors

author

Agnieszka Kraj

• Neurobiochemistry Unit, Faculty of Chemistry and Regional Laboratory, Jagiellonian University, Kraków, Poland

author

Tomasz Dylag

• Neurobiochemistry Unit, Faculty of Chemistry and Regional Laboratory, Jagiellonian University, Kraków, Poland

author

Anna Gorecka-Drzazga

• >Department of Microsystem Electronics and Photonics, Wrocław University of Technology, Wrocław, Poland

author

Sylwester Bargiel

• >Department of Microsystem Electronics and Photonics, Wrocław University of Technology, Wrocław, Poland

author

Jan Dziuban

• >Department of Microsystem Electronics and Photonics, Wrocław University of Technology, Wrocław, Poland

author

Jerzy Silberring

• Center for Polymer Chemistry, Polish Academy of Sciences, Zabrze, Poland

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