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2003 | 50 | 3 | 783-787
Article title

Desorption/ionization on silicon for small molecules: a promising alternative to MALDI TOF.

Content
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Languages of publication
EN
Abstracts
EN
A method has been developed for laser desorption/ionization of catecholamines from porous silicon. This methodology is particularly attractive for analysis of small molecules. MALDI TOF mass spectrometry, although a very sensitive technique, utilizes matrices that need to be mixed with the sample prior to their analysis. Each matrix produces its own background, particularly in the low-molecular mass region. Therefore, detection and identification of molecules below 400 Da can be difficult. Desorption/ionization of samples deposited on porous silicon does not require addition of a matrix, thus, spectra in the low-molecular mass region can be clearly readable. Here, we describe a method for the analysis of catecholamines. While MALDI TOF is superior for proteomics/peptidomics, desorption/ionization from porous silicon can extend the operating range of a mass spectrometer for studies on metabolomics (small organic molecules and their metabolites, such as chemical neurotransmitters, prostaglandins, steroids, etc.).
Publisher

Year
Volume
50
Issue
3
Pages
783-787
Physical description
Dates
published
2003
received
2003-05-30
revised
2003-08-23
accepted
2003-09-09
Contributors
  • Neurobiochemistry Unit, Faculty of Chemistry and Regional Laboratory, Jagiellonian University, Kraków, Poland
author
  • Neurobiochemistry Unit, Faculty of Chemistry and Regional Laboratory, Jagiellonian University, Kraków, Poland
  • >Department of Microsystem Electronics and Photonics, Wrocław University of Technology, Wrocław, Poland
  • >Department of Microsystem Electronics and Photonics, Wrocław University of Technology, Wrocław, Poland
author
  • >Department of Microsystem Electronics and Photonics, Wrocław University of Technology, Wrocław, Poland
  • Center for Polymer Chemistry, Polish Academy of Sciences, Zabrze, Poland
References
  • Andersen JS, Svensson B, Roepstorff P. (1996) Electrospray ionization and matrix assisted laser desorption/ionization mass spectrometry: powerful analytical tools in recombinant protein chemistry. Nat Biotechnol.; 14: 449-57.
  • Bergquist J, Sciubisz A, Kaczor A, Silberring J. (2002) Catecholamines and methods for their identification and quantitation in biological tissues and fluids. J Neurosci Methods.; 113: 1-13.
  • Kowalski P, Stoerker J. (2000) Accelerating discoveries in the proteome and genome with MALDI TOF MS. Pharmacogenomics.; 1: 359-66.
  • Mann M, Hendrickson RC, Pandey A. (2001) Analysis of proteins and proteomes by mass spectrometry. Annu Rev Biochem.; 70: 437-73.
  • Nieradko Ł. (2001) Mikroelektroniczne metody modyfikacji właściwości separujących mikromechanicznych kapilarnych kolumn chromatograficznych. Ph.D. Thesis, Wrocław University of Technology, Department of Electronics (in Polish).
  • Shen Z, Thomas JJ, Averbuj C, Broo KM, Engelhard M, Crowell JE, Finn M.G, Siuzdak G. (2001) Porous silicon as a versatile platform for laser desorption/ionization mass spectrometry. Anal Chem.; 73: 612-9.
  • Thomas JJ, Shen Z, Crowell JE, Finn MG, Siuzdak G. (2001) Desorption/ionization on silicon (DIOS): a diverse mass spectrometry platform for protein characterization. Proc Natl Acad Sci U S A.; 98: 4932-7.
  • Wei J, Buriak JM, Siuzdak G. (1999) Desorption-ionization mass spectrometry on porous silicon. Nature.; 399: 243-6.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv50i3p783kz
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