Prostaglandin-J2 upregulates expression of matrix metalloproteinase-1 independently of activation of peroxisome proliferator-activated receptor-γ.

• Authors: Alicja Józkowicz , Ihor Huk , Anneliese Nigisch , Jarosław Cisowski , Guenter Weigel , Józef Dulak
• Languages of publication: EN

Abstracts

EN

Peroxisome proliferator-activated receptor-γ (PPARγ) is a ligand-inducible nuclear receptor that functions as a transcription factor involved in lipid metabolism, inflammatory response and angiogenesis. The most potent endogenous PPARγ activator is 15-deoxy-Δ12,14prostaglandin-J2 (15d-PGJ2), whereas synthetic ligands include the oral antidiabetic drugs thiazolidinediones (TZDs). Activation of PPARγ was reported to decrease the synthesis of matrix metalloproteinases (MMPs) in vascular smooth muscle cells and macrophages. We aimed to investigate the effect of PPARγ ligands on expression of MMP-1 and urokinase plasminogen activator (uPA) in human microvascular endothelial cells (HMEC-1). We found that treatment of HMEC-1 with 15d-PGJ2 increased the synthesis of MMP-1 protein up to 168% comparing to untreated cells. TZDs (ciglitazone and troglitazone), more potent activators of PPARγ in HMEC-1, did not influence MMP-1 production, arguing against the involvement of PPARγ in this process. Importantly, the stimulatory effect of 15d-PGJ2 was reversed by the antioxidant N-acetyl-cysteine (NAC), suggesting a contribution of oxidative stress. We demonstrated also that 15d-PGJ2 did not change the activity of MMP-1 promoter, but increased the stability of MMP-1 mRNA. In contrast, 15d-PGJ2 very potently inhibited the synthesis of uPA. This effect was in part mimicked by ciglitazone and troglitazone implying an involvement of PPARγ. Accordingly, NAC did not modify the inhibitory effect of 15d-PGJ2 on uPA expression. In conclusion, we postulate that 15d-PGJ2 may differently regulate the synthesis of proteases involved in angiogenesis : it upregulates MMP-1 expression in HMEC-1 through induction of oxidative stress, and inhibits uPA synthesis partly by activation of PPARγ.

Keywords

EN

matrix metalloproteinase-1; urokinase plasminogen activator; prostaglandin-J2; peroxisome proliferator-activated receptor-γ; endothelial cells

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2003
• Volume: 50
• Issue: 3
• Pages: 677-689

Dates

published

2003

received

2003-05-30

revised

2003-08-12

accepted

2003-09-05

Contributors

author

Alicja Józkowicz

• Department of Molecular Genetics, Faculty of Biotechnology, Jagiellonian University, Kraków, Poland

author

Ihor Huk

• Department of Vascular Surgery, University of Vienna, Vienna, Austria

author

Anneliese Nigisch

• Department of Cardiothoracic Surgery, University of Vienna, Vienna, Austria

author

Jarosław Cisowski

• Department of Cell Biochemistry, Faculty of Biotechnology, Jagiellonian University, Kraków, Poland

author

Guenter Weigel

• Department of Cardiothoracic Surgery, University of Vienna, Vienna, Austria

author

Józef Dulak

• Department of Cell Biochemistry, Faculty of Biotechnology, Jagiellonian University, Kraków, Poland

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