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2003 | 50 | 2 | 567-572
Article title

Evidence for essential histidine and dicarboxylic amino-acid residues in the active site of UDP-glucose : solasodine glucosyltransferase from eggplant leaves.

Content
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Languages of publication
EN
Abstracts
EN
Effects of several chemical probes selectively modifying various amino-acid residues on the activity of UDP-glucose : solasodine glucosyltransferase from eggplant leaves was studied. It was shown that diethylpyrocarbonate (DEPC), a specific modifier of histidine residues, was strongly inhibitory. However, in the presence of excessive amounts of the enzyme substrates, i.e. either UDP-glucose or solasodine, the inhibitory effect of DEPC was much weaker indicating that histidine (or histidines) are present in the active site of the enzyme. Our results suggest also that unmodified residues of glutamic (or aspartic) acid, lysine, cysteine, tyrosine and tryptophan are necessary for full activity of the enzyme. Reagents modifying serine and arginine residues have no effect on the enzyme activity.
Publisher

Year
Volume
50
Issue
2
Pages
567-572
Physical description
Dates
published
2003
accepted
2002-06-02
received
2003-04-08
revised
2003-05-27
Contributors
  • Institute of Biochemistry, Warsaw University, Warszawa, Poland
  • Institute of Biochemistry, Warsaw University, Warszawa, Poland
  • Institute of Biochemistry, Warsaw University, Warszawa, Poland
  • Institute of Biochemistry, Warsaw University, Warszawa, Poland
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv50i2p567kz
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