Cellular level of 8-oxo-2'-deoxyguanosine in DNA does not correlate with urinary excretion of the modified base/nucleoside.

• Authors: Marek Foksinski , Daniel Gackowski , Rafał Rozalski , Ryszard Olinski
• Languages of publication: EN

Abstracts

EN

We assessed a relationship between the level of 8-oxodG in leukocyte DNA measured with the high performance liquid chromatography with electrochemical detection (HPLC/EC) technique and urinary excretion of the modified nucleoside/base analysed with a recently developed methodology involving HPLC prepurification followed by gas chromatography with isotope dilution mass spectrometric detection. No correlation was found between these markers of oxidative DNA damage commonly used in epidemiological studies. Several possible explanations of this finding are discussed.

Keywords

EN

8-oxoguanine; oxidative DNA damage; 8-oxo-2'-deoxyguanosine

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2003
• Volume: 50
• Issue: 2
• Pages: 549-553

Dates

published

2003

received

2003-02-12

revised

2003-05-21

accepted

2003-05-30

Contributors

author

Marek Foksinski

• Department of Clinical Biochemistry, The Ludwik Rydygier Medical University in Bydgoszcz, Bydgoszcz, Poland

author

Daniel Gackowski

• Department of Clinical Biochemistry, The Ludwik Rydygier Medical University in Bydgoszcz, Bydgoszcz, Poland

author

Rafał Rozalski

• Department of Clinical Biochemistry, The Ludwik Rydygier Medical University in Bydgoszcz, Bydgoszcz, Poland

author

Ryszard Olinski

• Department of Clinical Biochemistry, The Ludwik Rydygier Medical University in Bydgoszcz, Bydgoszcz, Poland

References

• Cheng KC, Cahill DS, Kasai H, Nishimura S, Loeb LA. (1992) J Biol Chem.; 267: 166-72.
• Collins AR. (1999) Bioessays.; 21: 238-46.
• Collins AR, Gedik CM, Olmedilla B, Southon S, Bellizzi M. (1998) FASEB J.; 12: 1397-400.
• Cooke MS, Evans MD, Herbert KE, Lunec J. (2000) Free Radic Res.; 32: 381-97.
• Devanaboyina U, Gupta RC. (1996) Carcinogenesis.; 17: 917-24.
• Dizdaroglu M. (1994) Methods Enzymol.; 234: 3-16.
• ESCODD. (2000) Free Radic Res.; 32: 333-41.
• ESCODD. (2002a) Carcinogenesis.; 23: 2129-33.
• ESCODD. (2002b) Free Radic Res.; 36: 239-45.
• Feig DI, Reid TM, Loeb LA. (1994) Cancer Res.; 54: 1890s-4s.
• Floyd RA. (1990) Carcinogenesis.; 11: 1447-50.
• Foksinski M, Bialkowski K, Skiba M, Ponikowska I, Szmurlo W, Olinski R. (1999) Mutat Res.; 438: 23-7.
• Foksinski M, Kotzbach R, Szymanski W, Olinski R. (2000) Free Radic Biol Med.; 29: 597-601.
• Gackowski D, Ciecierski M, Jawien A, Olinski R. (2001a) Acta Biochim Polon.; 48: 535-9.
• Gackowski D, Rozalski R, Roszkowski K, Jawien A, Foksinski M, Olinski R. (2001b) Free Radic Res.; 35: 825-32.
• Gackowski D, Kruszewski M, Bartlomiejczyk T, Jawien A, Ciecierski M, Olinski R. (2002) J Biol Inorg Chem.; 7: 548-50.
• Halliwell B, Gutteridge JM. (1999) Oxford University Press, New York, NY.
• Hayakawa H, Taketomi A, Sakumi K, Kuwano M, Sekiguchi M. (1995) Biochemistry.; 34: 89-95.
• Kasai H. (1997) Mutat Res.; 387: 147-63.
• Lenton KJ, Therriault H, Fulop T, Payette H, Wagner JR. (1999) Carcinogenesis.; 20: 607-13.
• Lindahl T. (1993) Nature.; 362: 709-15.
• Loft S, Poulsen HE. (1998) Acta Biochim Polon.; 45: 133-44.
• Loft S, Astrup A, Buemann B, Poulsen HE. (1994) FASEB J.; 8: 534-7.
• Miller SA, Dykes DD, Polesky HF. (1988) Nucleic Acids Res.; 16: 1215.
• Nakae D, Akai H, Kishida H, Kusuoka O, Tsutsumi M, Konishi Y. (2000) Lab Invest.; 80: 249-61.
• Olinski R, Gackowski D, Foksinski M, Rozalski R, Roszkowski K, Jaruga P. (2002) Free Radic Biol Med.; 33: 192-200.
• Rozalski R, Gackowski D, Roszkowski K, Foksinski M, Olinski R. (2002) Cancer Epidemiol Biomarkers Prev.; 11: 1072-5.
• Shigenaga MK, Gimeno CJ, Ames BN. (1989) Proc Natl Acad Sci U S A.; 86: 9697-701.