Overproduction and purification of the CcpA protein from Lactococcus lactis.

• Authors: Magdalena Kowalczyk , Jacek Bardowski
• Languages of publication: EN

Abstracts

EN

In this work we present cloning and overexpression of lactococcal CcpA protein in Escherichia coli Xl1blue strain as a fusion with 6×His tag. A high yield of the CcpA protein was obtained when the cells were cultured in liquid medium LB with 100 Μg/ml ampicillin at 37°C and subsequently for 4 h after induction by IPTG. The procedure let us obtain 5 mg of homogenous CcpA protein. Glutaraldehyde crosslinking analysis indicated the formation of dimer or tetramer forms of the CcpA protein.

Keywords

EN

His-tag; cross links; dimers; CcpA

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2003
• Volume: 50
• Issue: 2
• Pages: 455-459

Dates

published

2003

received

2003-05-12

accepted

2003-05-21

Contributors

author

Magdalena Kowalczyk

• Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics PAS, Warszawa, Poland

author

Jacek Bardowski

• Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics PAS, Warszawa, Poland

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