PL EN


Preferences help
enabled [disable] Abstract
Number of results
2003 | 50 | 2 | 455-459
Article title

Overproduction and purification of the CcpA protein from Lactococcus lactis.

Content
Title variants
Languages of publication
EN
Abstracts
EN
In this work we present cloning and overexpression of lactococcal CcpA protein in Escherichia coli Xl1blue strain as a fusion with 6×His tag. A high yield of the CcpA protein was obtained when the cells were cultured in liquid medium LB with 100 Μg/ml ampicillin at 37°C and subsequently for 4 h after induction by IPTG. The procedure let us obtain 5 mg of homogenous CcpA protein. Glutaraldehyde crosslinking analysis indicated the formation of dimer or tetramer forms of the CcpA protein.
Keywords
EN
Publisher

Year
Volume
50
Issue
2
Pages
455-459
Physical description
Dates
published
2003
received
2003-05-12
accepted
2003-05-21
Contributors
  • Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics PAS, Warszawa, Poland
  • Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics PAS, Warszawa, Poland
References
  • Aleksandrzak T, Kowalczyk M, Kok J, Bardowski J. (2000) Regulation of carbon catabolism in Lactococcus lactis. In Food Biotechnology; Bielecki S, Tramper J, Polak J. eds, 17: 61-6. Elsevier Science B.V.
  • Bradford MM. (1976) Anal Biochem.; 72: 248-54.
  • Deutscher J, Reizer J, Fischer C, Galinier A, Saier MH, Jr, Steinmetz M. (1994) J Bacteriol.; 176: 3336-44.
  • Deutscher J, Kuster E, Bergstedt U, Charrier V, Hillen W. (1995) Mol Microbiol.; 15:1049-53.
  • Gibson TJ. (1984) Studies on the Epstein-Barr virus genome. Ph.D. Thesis.; Cambridge University Press.
  • Gosseringer R, Kuster E, Galinier A, Deutscher J, Hillen W. (1997) J Mol Biol.; 266: 665-76.
  • Guedon E, Jamet E, Renault P. (2002) Antonie Van Leeuwenhoek.; 82: 93-112.
  • Mahr K, Esteban CD, Hillen W, Titgemeyer F, Perez-Martinez G. (2002) J Mol Microbiol Biotechnol., 4: 489-94.
  • Miwa Y, Saikawa M, Fujita Y. (1994) Microbiology.; 140: 2567-75.
  • Sambrook J, Fritsch EF, Maniatis T. (1989) Molecular cloning a laboratory manual. Cold Spring Harbor Laboratory Press.
  • Sanger F, Nicklen S, Coulson AR. (1977) Proc Natl Acad Sci U S A.; 74: 5463-7.
  • Schick J, Weber B, Klein JR, Henrich B. (1999) Microbiology.; 145: 3147-54.
  • Tebbe J, Orth P, Kuster-Schock EK, Hillen W, Saenger W, Hinrichs W. (2000) Acta Crystallogr D Biol Crystallogr.; 56: 67-9.
  • Titgemeyer F, Hillen W. (2002) Antonie Van Leeuwenhoek.; 82: 59-71.
  • Weickert MJ, Chambliss GJ. (1990) Proc Natl Acad Sci U S A.; 87: 6238-42.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv50i2p455kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.