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2003 | 50 | 2 | 405-413
Article title

Coenzyme Q releases the inhibitory effect of free fatty acids on mitochondrial glycerophosphate dehydrogenase.

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Data presented in this paper show that the size of the endogenous coenzyme Q (CoQ) pool is not a limiting factor in the activation of mitochondrial glycerophosphate-dependent respiration by exogenous CoQ3, since successive additions of succinate and NADH to brown adipose tissue mitochondria further increase the rate of oxygen uptake. Because the inhibition of glycerophosphate-dependent respiration by oleate was eliminated by added CoQ3, our data indicate that the activating effect of CoQ3 is related to the release of the inhibitory effect of endogenous free fatty acids (FFA). Both the inhibitory effect of FFA and the activating effect of CoQ3 could be demonstrated only for glycerophosphate-dependent respiration, while succinate- or NADH-dependent respiration was not affected. The presented data suggest differences between mitochondrial glycerophosphate dehydrogenase and succinate or NADH dehydrogenases in the transfer of reducing equivalents to the CoQ pool.
Physical description
  • Center for Experimental Cardiovascular Research, Czech Academy of Sciences, Prague, Czech Republic,
  • Center for Molecular Genomics of the Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic,
  • Department of Biochemistry and Microbiology, Instiute Chem. Technology Prague, Prague, Czech Republic
  • Department of Biochemistry, Medical School, University of Bologna, Bologna, Italy
  • Department of Biochemistry, Medical School, University of Bologna, Bologna, Italy
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