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2003 | 50 | 1 | 249-253
Article title

Analysis of the G/C polymorphism in the 5'-untranslated region of the RAD51 gene in breast cancer.

Content
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Languages of publication
EN
Abstracts
EN
The breast cancer suppressor proteins BRCA1 and BRCA2 interact with RAD51, a protein essential for maintaining genomic stability by playing a central role in homology-dependent recombinational repair of the DNA double-strand breaks. Therefore, genetic variability in the RAD51 gene may contribute to the appearance and/or progression of breast cancer. A single nucleotide polymorphism in the 5'- untranslated region of RAD51 (a G to C substitution at position 135, the G/C polymorphism) is reported to modulate breast cancer risk. We investigated the distribution of genotypes and frequency of alleles of the G/C polymorphism in breast cancer. Tumor tissues were obtained from postmenopausal women with node-negative and node-positive breast carcinoma with uniform tumor size. Blood samples from age matched healthy women served as control. The G/C polymorphism was determined by PCR-based MvaI restriction fragment length polymorphism. The distribution of the genotypes of the G/C polymorphism did not differ significantly (P >0.05) from those predicted by the Hardy-Weinberg distribution. There were no differences in the genotype distribution and allele frequencies between node-positive and node-negative patients. There were no significant differences between distributions of the genotypes in subgroups assigned to histological grades according to Scarf-Bloom-Richardson criteria and the distribution predicted by Hardy-Weinberg equilibrium (P >0.05). Our study implies that the G/C polymorphism of the RAD51 gene may not be directly involved in the development and/or progression of breast cancer and so it may not be useful as an independent marker in this disease.
Publisher

Year
Volume
50
Issue
1
Pages
249-253
Physical description
Dates
published
2003
(unknown)
2002-08-08
revised
2003-02-27
accepted
2003-03-10
Contributors
  • Department of Molecular Genetics, University of Lodz, Łódź, Poland
  • Department of Molecular Genetics, University of Lodz, Łódź, Poland
  • Department of Molecular Genetics, University of Lodz, Łódź, Poland
  • Polish Mother's Memorial Research Institute, Łódź, Poland
  • Polish Mother's Memorial Research Institute, Łódź, Poland
author
  • Department of Oncological Surgery, N. Copernicus Hospital, Łódź, Poland
  • Department of Oncological Surgery, N. Copernicus Hospital, Łódź, Poland
  • Department of Oncological Surgery, N. Copernicus Hospital, Łódź, Poland
  • Department of Clinical Pharmacology, Medical University of Lodz, Łódź, Poland
References
  • Chen PL, Chen CF, Chen Y, Xiao J, Sharp ZD, Lee WH. (1998) Proc Natl Acad Sci U S A.; 95: 5287-92.
  • Gonzales R, Silva JM, Dominguez G, Garcia JM, Martinez G, Vargas J, Provencio M, Espana P, Bonilla F. (1999) Br J Cancer.; 81: 503-9.
  • Kute TE, Grondahl-Hansen J, Shao SM, Long M, Russel G, Brunner N. (1998) Breast Cancer Res Treat.; 47: 9-16.
  • Levy-Lahad E, Lahad A, Eisenberg S, Dagan E, Paperna T, Kasinetz L, Catane R, Kaufman B, Beller U, Renbaum P, Gershoni-Baruch R. (2001) Proc Natl Acad Sci U S A.; 98: 3232-6.
  • Wang W, Tucker MA, Doody MM, Tarone RE, Struewing JP. (1999) Am J Hum Genet.; 655: 22 (abstract).
  • Wang WW, Spurdle A, Kolachana P, Bove B, Modan B, Ebbers SM, Suthers G, Tucker MA, Kaufman DJ, Doody MM, Tarone RE, Daly M, Levavi H, Pierce H, Chetrit A, Yechezkel GH, Chenevix-Trench G, Offit K, Godwin AK, Struewing JP. (2001) Cancer Epidemiol Biomarkers Prev.; 10: 955-60.
  • Welcsh PL, Owens KN, King MC. (2000) Trends Genet.; 16: 69-74.
  • Yoshikawa K, Ogawa T, Baer R, Hemmi H, Honda K, Yamauchi A, Inamoto T, Ko K, Yazumi S, Motoda H, Kodama H, Noguchi S, Gazdar AF, Yamaoka Y, Takahashi R. (2000) Int J Cancer.; 88: 28-36.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv50i1p249kz
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