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2003 | 50 | 1 | 191-195
Article title

Hyperthermia can differentially modulate the repair of doxorubicin-damaged DNA in normal and cancer cells*.

Content
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EN
Abstracts
EN
Hyperthermia can modulate the action of many anticancer drugs, and DNA repair processes are temperature-dependent, but the character of this dependence in cancer and normal cells is largely unknown. This subject seems to be worth studying, because hyperthermia can assist cancer therapy. A 1-h incubation at 37°C of normal human peripheral blood lymphocytes and human myelogenous leukemia cell line K562 with 0.5 μM doxorubicin gave significant level of DNA damage as assessed by the alkaline comet assay. The cells were then incubated in doxorubicin-free repair medium at 37°C or 41°C. The lymphocytes incubated at 37°C needed about 60 min to remove completely the damage to their DNA, whereas at 41°C the time required for complete repair was shortened to 30 min. There was also a difference between the repair kinetics at 37°C and 41°C in cancer cells. Moreover, the kinetics were different in doxorubicin-sensitive and resistant cells. Therefore, hyperthermia may significantly affect the kinetics of DNA repair in drug-treated cells, but the magnitude of the effect may be different in normal and cancer cells. These features may be exploited in cancer chemotherapy to increase the effectiveness of the treatment and reduce unwanted effects of anticancer drugs in normal cells and fight DNA repair-based drug resistance of cancer cells.
Publisher

Year
Volume
50
Issue
1
Pages
191-195
Physical description
Dates
published
2003
received
2002-10-09
accepted
2003-01-27
Contributors
  • Department of Molecular Genetics, University of Lodz, Łódź, Poland
author
  • Department of Molecular Genetics, University of Lodz, Łódź, Poland
  • Polish Mother's Memorial Research Institute, Łódź, Poland
References
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  • Majsterek I, Blasiak J, Mlynarski W, Hoser G, Skorski T. (2002) Cell Biol Int.; 26: 363-70.
  • Singh NP, McCoy MT, Tice RR, Schneider EL. (1988) Exp Cell Res.; 175: 184-91.
  • Slupianek A, Hoser G, Majsterek I, Bronisz A, Malecki M, Blasiak J, Fishel R, Skorski T. (2002) Mol Cell Biol.; 22: 4189-201.
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv50i1p191kz
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