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2003 | 50 | 1 | 181-190
Article title

DNA damage and repair in lymphocytes of normal individuals and cancer patients: studies by the comet assay and micronucleus tests.

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EN
Abstracts
EN
A population study is reported in which the DNA damage induced by γ-radiation (2 Gy) and the kinetics of the subsequent repair were estimated by the comet and micronucleus assays in isolated lymphocytes of 82 healthy donors and patients with head and neck cancer before radiotherapy. The parameters of background and radiation-induced DNA damage, rate of repair, and residual non-repaired damage were measured by comet assay, and the repair kinetics for every donor were computer-fitted to an exponential curve. The level of background DNA damage before irradiation measured by comet assay as well as the level of micronuclei were significantly higher in the head and neck cancer patient group than in the healthy donors, while the parameters of repair were widely scattered in both groups. Cancer patient group contained significantly more individuals, whose irradiated lymphocytes showed high DNA damage, low repair rate and high non-repaired DNA damage level. Lymphocytes of donors belonging to this subgroup showed significantly lower inhibition of cell cycle after irradiation.
Publisher

Year
Volume
50
Issue
1
Pages
181-190
Physical description
Dates
published
2003
received
2002-10-09
revised
2002-11-29
accepted
2003-01-17
Contributors
  • Institute of Cell Biology, National Academy of Sciences, Lviv, Ukraine
  • Institute of Automatic Control, Silesian University of Technology, Gliwice, Poland
  • Department of Experimental and Clinical Radiobiology, Center of Oncology-M. Skłodowska-Curie Memorial Institute, Gliwice, Poland
  • Department of Experimental and Clinical Radiobiology, Center of Oncology-M. Skłodowska-Curie Memorial Institute, Gliwice, Poland
References
  • Brammer I, Zoller M, Dikomey E. (2001) Relationship between cellular radiosensitivity and DNA damage measured by comet assay in human normal, NBS and AT fibroblasts. Int J Radiat Biol.; 77: 929-38.
  • Breen AP, Murphy JA. (1995) Reactions of oxyl radicals with DNA. Free Radic Biol Med.; 18: 1033-77.
  • Cheng L, Sturgis EM, Eicher SA, Spitz MR, Wei Q. (2002) Expression of nucleotide excision repair genes and the risk for squamous cell carcinoma of the head and neck. Cancer.; 94: 393-7.
  • Collins AR, Dusinska M, Franclin M, Somorovska M, Petrovska H, Duthie S, Fillion L, Panayiotidis M, Raslova K, Vaughan N. (1997) Comet assay in human biomonitoring studies: reliability, validation and applications. Environ Mol Mutagen.; 30: 139-46.
  • Elston RC, Johnston WD. (1994) In Essentials of Biostatistics. Davis, ed., Philadelphia.
  • Fairbairn DW, Olive PL, O'Neill KL. (1995) The comet assay: a comprehensive review. Mutat Res.; 339: 37-59.
  • Fenech MF. (1993) The cytokinesis-block micronucleus technique and its application to genotoxicity studies in human populations. Environ Health Perspect.; 101: 101-7.
  • Green M, Low J, Harcourt SA, Akinluyi P, Row T, Cole J, Anstey AV, Arlett CV. (1992) UV-C sensitivity of unstimulated and stimulated human lymphocytes from normal and Xeroderma pigmentosum donors in the comet assay: a potential diagnostic technique. Mutat Res.; 273: 137-44.
  • Kapiszewska M, Szumiel I, Lange Damage at two levels of DNA folding measured by fluorescent halo technique in X-irradiated L5178Y-R and L5178Y-S cells.+II.+Repair.+ Radiat Environ Biophys.; 33: 35-44.
  • Keshava C, Nagalakshmi R, Ong T. (1996) Inhibitory effect of folinic acid on radiation-induced micronuclei and chromosomal aberrations in V79 cells. Mutat Res.; 352: 123-34.
  • Louagie H, Cornelissen M, Philippe J, Vral A, Thierens H, De Ridder L. (1998) Flow cytometric scoring of apoptosis compared to electron microscopy in gamma irradiated lymphocytes. Cell Biol Int.; 22: 277-83.
  • Maciejewski B. (1997) Future directions for radiotherapy: gray or blue perspective? Nowotwory.; 47: 467-82.
  • Miyagi Y, Zhang H, Wheeler KT. (1997) Radiation-induced DNA damage in tumors and normal tissues: IV. Influence of proliferation status and cell type on the formation of oxygen-dependent DNA damage in cultured cells. Radiat Res.; 148: 29-34.
  • Nascimento PA, da Silva MA, Oliviera EM, Suzuki M, Okazaki K. (2001) Evaluation of radioinduced damage and repair capacity in blood lymphocytes of breast cancer patients. Braz J Med Biol Res.; 34: 165-76.
  • Oleinick N, Balasubramaniam U, Xue L, Chiu S. (1994) Nuclear structure and the microdistribution of radiation damage in DNA. Int J Radiat Biol.; 66: 523-9.
  • Olive PL. (1992) DNA organization affects cellular radiosensitivity and detection of initial DNA strand breaks. Int J Radiat Biol.; 62: 389-96.
  • Olive PL. (1999) DNA damage and repair in individual cells: applications of the comet assay in radiobiology. Int J Radiat Biol.; 75: 395-405.
  • Olive PL., Banath JP, Durand RE. (1990) Detection of etoposide resistance by measuring DNA damage in individual Chinese hamster cells. J Natl Cancer Inst.; 82: 779-83.
  • Palyvoda O, Mukalov I, Polanska J, Wygoda A, Drobot L, Widel M, Rzeszowska-Wolny J. (2002) Radiation-induced DNA damage and its repair in lymphocytes of patients with head and neck cancer and healthy donors. Anticancer Res.; 22: 1721-6.
  • Powell S, Suit HD. (2000) Radiation biology for radiation oncologist. In Clinical radiation oncology: indications, techniques and results. Wang CC., ed, pp 46-8. Wiley&-Liss Inc.
  • Schmidt-Ullrich RK, Dent P, Grant S, Mikkelsen RB, Valerie K. (2000) Signal transduction and cellular radiation responses. Radiat Res.; 153: 245-57.
  • Sokal RR, Rohlf FJ. (1997) Biometry. The principles and practice of statistics in biological research. 3rd edn. W.H. Freeman and Co. New York.
  • Sturgis EM, Zheng R, Li L, Castillo EJ, Eiher SA, Chen M, Strom SS, Spitz MR, Wei Q. (2000) XPD/ERCC2 polymorphisms and risk of head and neck cancer: a case-control analysis. Carcinogenesis.; 21: 2219-23.
  • Sturgis EM, Castillo EJ, Eiher SA, Strom SS, Spitz MR, Wei Q. (2002) XPD/ERCC2 exon 8 polymorphisms: rarity and luck of significance in risk of squamous cell carcinoma of the head and neck. Oral Oncol.; 38: 475-7.
  • Sturgis EM, Dahlstrom KR, Spitz MR, Wei Q. (2002) DNA repair gene ERCC1 and ERCC2/XPD polymorphisms and risk of squamous cell carcinoma of the head and neck. Arch Otolaryngol Head Neck Surg.; 128: 1084-8.
  • Terris DJ, Ho EY, Ibrahim HZ, Dorie MJ, Kovacs MS, Le QT, Koong AC, Pinto HA, Brown JM. (2002) Estimating DNA repair by sequential evaluation of head and neck tumor radiation sensitivity using the comet assay. Arch Otolaryngol Head Neck Surg.; 128: 698-702.
  • Wojewodzka M, Kruszewski M, Szumiel I. (1997) Effect of signal transduction inhibition in adapted lymphocytes: micronuclei frequency and DNA repair. Int J Radiat Biol.; 71: 245-52.
  • Wuttke K, Streffer C, Muller W. (1993) Radiation induced micronuclei in subpopulations of human lymphocytes. Mutat Res.; 286: 181-8.
  • Wygoda A, Skladowski K. (1999) Risk factors for the toxicity of radiation treatment of head and neck cancer. Nowotwory.; 49: 7-13.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv50i1p181kz
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