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2003 | 50 | 1 | 139-144
Article title

Defective Brca2 influences topoisomerase I activity in mammalian cells*.

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EN
Abstracts
EN
The Chinese hamster cell mutant V-C8 is defective in the Brca2 gene (Kraakman-van der Zwet et al., 2002, Cell Biol.; 22: 669). Here we report that V-C8 cells were 10-fold more sensitive to camptothecin, an inhibitor of topoisomerase I, than the parental V79 cells. The level of the relaxation activity of topoisomerase I in nuclear extracts was also lower (4-fold) in V-C8 than V79 cells, in spite of the fact that the level of the topoisomerase I protein was the same in these cells. The survival of V-C8 cells in the presence of camptothecin, the sensitivity of V-C8 topoisomerase I to camptothecin, and the level of the relaxation activity in V-C8 nuclear extract were almost completely restored by transfection of V-C8 cells with the murine Brca2 gene or by the transfer of human chromosome 13 providing the BRCA2 gene. These results indicate that the observed changes in the topoisomerase I activity in V-C8 are due to the defective function of the Brca2 gene.
Year
Volume
50
Issue
1
Pages
139-144
Physical description
Dates
published
2003
received
2003-01-13
revised
2003-02-17
accepted
2003-03-05
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv50i1p139kz
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