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2002 | 49 | 4 | 937-945
Article title

Mouse cytosolic acetyl-CoA hydrolase, a novel candidate for a key enzyme involved in fat metabolism: cDNA cloning, sequencing and functional expression.

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EN
Abstracts
EN
A cytosolic acetyl-CoA hydrolase (CACH) cDNA has been isolated from mouse liver cDNA library and sequenced. Recombinant expression of the cDNA in insect cells resulted in overproduction of active acetyl-CoA hydrolyzing enzyme protein. The mouse CACH cDNA encoded a 556-amino-acid sequence that was 93.5% identical to rat CACH, suggesting a conserved role for this enzyme in the mammalian liver. Database searching shows no homology to other known proteins, but reveals homological cDNA sequences showing two single-nucleotide polymorphisms (SNPs) in the CACH coding region. The discovery of mouse CACH cDNA is an important step towards genetic studies on the functional analysis of this enzyme by gene-knockout and transgenic approaches.
Publisher

Year
Volume
49
Issue
4
Pages
937-945
Physical description
Dates
published
2002
received
2002-04-30
revised
2002-09-25
accepted
2002-10-03
Contributors
  • Department of Biochemistry, St. Marianna University School of Medicine, Kanagawa, Kanaga, Japan
  • Department of Biochemistry, St. Marianna University School of Medicine, Kanagawa, Kanaga, Japan
  • Department of Biochemistry, St. Marianna University School of Medicine, Kanagawa, Kanaga, Japan
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv49i4p937kz
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