PL EN


Preferences help
enabled [disable] Abstract
Number of results
2002 | 49 | 4 | 907-916
Article title

Protective role of L-methionine against free radical damage of rat brain synaptosomes

Content
Title variants
Languages of publication
EN
Abstracts
EN
Incubation of rat brain synaptosomal/mitochondrial fraction with tert-butylhydroperoxide resulted in accumulation of the lipid peroxidation product, conjugated dienes, damage of the synaptosomal membrane as evidenced by leakage of lactate dehydrogenase, and decrease of the total content of glutathione and of the GSH/GSSG ratio. This treatment also produced a considerable decrease of the ouabain-sensitive ATPase activity and a much smaller diminution of the activities of glutathione reductase and glutathione transferase. Preincubation of the synaptosomal/mitochondrial fraction with 0.5 or 1.0 mM L-methionine significantly protected against lipid peroxidation, membrane damage and changes in the glutathione system produced by low (1 mM) concentrations of tert-butylhydroperoxide and completely prevented inactivation of ouabain-sensitive ATPase, glutathione reductase and glutathione transferase by such treatment. The importance of L-methionine in antioxidant protection is discussed.
Year
Volume
49
Issue
4
Pages
907-916
Physical description
Dates
published
2002
received
2002-11-12
accepted
2002-12-02
References
  • Aebi H. (1984) Catalase in vitro. Methods Enzymol.; 105: 121-6.
  • Akerboom TPM, Sies H. (1981) Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples. Methods Enzymol.; 77: 373-82.
  • Arkhipenko YuV, Meerson FZ, Sazontova TG, Kagan VE. (1985) Mode of lipid peroxidation-induced inhibition of Na,K-ATPase. Acta Physiol Pharmacol Bulg.; 11: 70-8.
  • Asano T, Koide T, Gotoh O, Joshita H, Hanamura T, Shigeno T, Takakura K. (1989) The role of free radicals and eicosanoids in the pathogenetic mechanism underlying ischemic brain edema. Mol Chem Neuropathol.; 10: 101-33.
  • Bagchi D, Vuchetich PJ, Bagchi M, Tran MX, Krohn RL, Ray SD, Stohs SJ. (1998) Protective effects of zinc salts on TPA-induced hepatic and brain lipid peroxidation, glutathione depletion, DNA damage and peritoneal macrophage activation in mice. Gen Pharmacol.; 30: 43-50.
  • Bartosz G, Fried R, Grzelinska E, Leyko W. (1977) Effect of hyperoxide radicals on bovine-erythrocyte membrane. Eur J Biochem.; 73: 261-4.
  • Berlett BS, Stadtman ER. (1997) Protein oxidation in aging, disease, and oxidative stress. J Biol Chem.; 272: 20313-6.
  • Canals S, Casarejos MJ, de Bernardo S, Rodriguez-Martin E, Mena MA. (2001) Glutathione depletion switches nitric oxide neurotrophic effects to cell death in midbrain cultures: implications for Parkinson's disease. J Neurochem.; 79: 1183-95.
  • Cardoso SM, Pereira C, Oliveira CR. (1998) The protective effect of vitamin E, idebenone and reduced glutathione on free radical mediated injury in rat brain synaptosomes. Biochem Biophys Res Commun.; 246: 703-10.
  • Carlberg I, Mannervik B. (1985) Glutathione reductase. Methods Enzymol.; 113: 484-90.
  • Carp H, Miller F, Hoidal JR, Janoff A. (1982) Potential mechanism of emphysema: alpha1-proteinase inhibitor recovered from lungs of cigarette smokers contains oxidized methionine and has decreased elastase inhibitory capacity. Proc Natl Acad Sci U S A.; 79: 2041-5.
  • Chumakova OV, Liopo AV. (1996) Acetylcholinesterase and choline uptake in striatum from rats with varying sleeping times. Alcohol Alcohol.; 31: 217-20.
  • Ciorba MA, Heinemann SH, Weissbach H, Brot N, Hoshi T. (1997) Modulation of potassium channel function by methionine oxidation and reduction. Proc Natl Acad Sci U S A.; 94: 9932-7.
  • De La Cruz JP, Pavia J, Gonzalez-Correa JA, Ortiz P, Sanchez de la Cuesta F. (2000) Effects of chronic administration of S-adenosyl-L-methionine on brain oxidative stress in rats. Naunyn Schmiedebergs Arch Pharmacol.; 361: 47-52.
  • Draczynska-Lusiak B, Doung A, Sun AY. (1998) Oxidized lipoproteins may play a role in neuronal cell death in Alzheimer disease. Mol Chem Neuropathol.; 33: 139-48.
  • Farber JL. (1994) Mechanisms of cell injury by activated oxygen species. Environ Health Perspect.; 102(Suppl. 10): 17-24.
  • Flohe L, Gunzler WA. (1984) Assays of glutathione peroxidase. Methods Enzymol.; 105: 114-21.
  • Foley TD. (1997) 5-HPETE is a potent inhibitor of neuronal Na+, K+-ATPase activity. Biochem Biophys Res Commun.; 235: 374-6.
  • Halliwell B, Gutteridge JMC. (1984) Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J.; 219: 1-14.
  • Hexum TD, Fried R. (1979) Effects of superoxide radicals on transport (Na+K) adenosine triphosphatase and protection by superoxide dismutase. Neurochem Res.; 4: 73-82.
  • Hitschke K, Buhler R, Apell HJ, Stark G. (1994) Inactivation of the Na,K-ATPase by radiation-induced free radicals. Evidence for a radical-chain mechanism. FEBS Lett.; 353: 297-300.
  • Huang WH, Wamg Y, Askari A. (1992) (Na++ K+)-ATPase: Inactivation and degradation induced by oxygen radicals. Int J Biochem.; 24: 621-6.
  • Iwata H, Iwata C, Matsuda T. (1988) Difference between two isozymes of (Na++ K+)-ATPase in the interaction with omeprazole. Jpn J Pharmacol.; 46: 35-42.
  • Jenner P. (1998) Oxidative mechanisms in nigral cell death in Parkinson's disease. Mov Disord.; 13: 24-34.
  • Keelan J, Allen NJ, Antcliffe D, Pal S, Duchen MR. (2001) Quantitative imaging of glutathione in hippocampal neurons and glia in culture using monochlorobimane. J Neurosci Res.; 66: 873-84.
  • Koide T, Asano T, Matsushita H, Takakura K. (1986) Enhancement of ATPase activity by a lipid peroxide of arachidonic acid in rat brain microvessels. J Neurochem.; 46: 235-42.
  • Lehotsky J, Kaplan P, Racay P, Matejovicova M, Drgova A, Mezesova V. (1999) Membrane ion transport systems during oxidative stress in rodent brain: protective effect of stobadine and other antioxidants. Life Sci.; 65: 1951-8.
  • Levine RL, Mosoni L, Berlett BS, Stadtman ER. (1996) Methionine residues as endogenous antioxidants in proteins. Proc Natl Acad Sci USA.; 93: 15036-40.
  • Levine RL, Berlett BS, Moskovitz J, Mosoni L, Stadtman ER. (1999) Methionine residues may protect proteins from critical oxidative damage. Mech Ageing Dev.; 107: 323-32.
  • Martinez-Cayuela M. (1995) Oxygen free radicals and human disease. Biochimie.; 77: 147-61.
  • Moskovitz J, Berlett BS, Poston JM, Stadtman ER. (1999) Methionine sulfoxide reductase in antioxidant defense. Methods Enzymol.; 300: 239-44.
  • Moskovitz J, Bar-Noy S, Williams WM, Requena J, Berlett BS, Stadtman ER. (2001) Methionine sulfoxide reductase (MsrA) is a regulator of antioxidant defense and lifespan in mammals. Proc Natl Acad Sci USA.; 98: 12920-5.
  • Palmer GC. (1987) Free radicals generated by xanthine oxidase-hypoxanthine damage adenylate cyclase and ATPase in gerbil cerebral cortex. Metab Brain Dis.; 2: 243-57.
  • Pocernich CB, La Fontaine M, Butterfield DA. (2000) In-vivo glutathione elevation protects against hydroxyl free radical-induced protein oxidation in rat brain. Neurochem Int.; 36: 185-91.
  • Reddy GB, Bhat KS. (1999) Protection against UVB inactivation ( in vitro) of rat lens enzymes by natural antioxidants. Mol Cell Biochem.; 194: 41-5.
  • Rice-Evans CA, Diplock AT, Symons MCR. (1991) Techniques in free radical research. Elsevier, Amsterdam.
  • Robyt JF, Ackerman RJ, Chittenden CG. (1971) Reaction of protein disulfide groups with Ellman's reagent: a case study of the number of sulfhydryl and disulfide groups in Aspergillus oryzae alpha-amylase, papain and lysozyme. Arch Biochem Biophys.; 147: 262-9.
  • Shukitt-Hale B, Erat SA, Joseph JA. (1998) Spatial learning and memory deficits induced by dopamine administration with decreased glutathione. Free Radic Biol Med.; 24: 1149-58.
  • Slyshenkov VS, Rakowska M, Moiseenok AG, Wojtczak L. (1995) Pantothenic acid and its derivatives protect Ehrlich ascites tumor cells against lipid peroxidation. Free Radic Biol Med.; 19: 767-72.
  • Slyshenkov VS, Moiseenok AG, Wojtczak L. (1996) Noxious effects of oxygen reactive species on energy coupling processes in Ehrlich ascites tumor mitochondria and the protection by pantothenic acid. Free Radic Biol Med.; 20: 793-800.
  • Stadtman ER, Moskovitz J, Berlett BS, Levine RL. (2002) Cyclic oxidation and reduction of protein methionine residues is an important antioxidant mechanism. Mol Cell Biochem.; 234-235: 3-9.
  • Swaim MW, Pizzo SV. (1988) Methionine sulfoxide and the oxidative regulation of plasma proteinase inhibitors. J Leukoc Biol.; 43: 365-79.
  • Urano S, Sato Y, Otonari T, Makabe S, Suzuki S, Ogata M, Endo T. (1998) Aging and oxidative stress in neurodegeneration. Biofactors.; 7: 103-12.
  • Vasault A. (1983) Lactate dehydrogenase: UV- method with pyruvate and NADH. In Methods of enzymatic analysis. Bergmeyer J, Grassl M. eds, 3rd edn., vol 3, pp 118-26. Verlage Chemie, Weinheim.
  • Villalobos MA, De La Cruz JP, Cuerda MA, Ortiz P, Smith-Agreda JM, Sanchez De La Cuesta F. (2000) Effect of S-adenosyl-L-methionine on rat brain oxidative stress damage in a combined model of permanent focal ischemia and global ischemia-reperfusion. Brain Res.; 883: 31-40.
  • Vogt W. (1995) Oxidation of methionyl residues in proteins: tools, targets, and reversal. Free Radic Biol Med.; 18: 93-105.
  • Weiler MH, Gundersen CB, Jenden DJ. (1981) Choline uptake and acetylcholine synthesis in synaptosomes: investigations using two different labeled variants of choline. J Neurochem.; 36: 1802-12.
  • Zeevalk GD, Bernard LP, Nicklas WJ. (1998) Role of oxidative stress and the glutathione system in loss of dopamine neurons due to impairment of energy metabolism. J Neurochem.; 70: 1421-30.
Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv49i4p907kz
Identifiers
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.