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2002 | 49 | 4 | 899-905
Article title

p19 detected in the rat retina and pineal gland is a guanylyl cyclase-activating protein (GCAP).

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The Ca2+-dependent activation of retina-specific guanylyl cyclase (retGC) is mediated by guanylyl cyclase-activating proteins (GCAPs). Here we report for the first time detection of a 19 kDa protein (p19) with GCAP properties in extracts of rat retina and pineal gland. Both extracts stimulate synthesis of cGMP in rod outer segment (ROS) membranes at low (30 nM) but not at high (1 mM) concentrations of Ca2+. At low Ca2+, immunoaffinity purified p19 activates guanylyl cyclase(s) in bovine ROS and rat retinal membranes. Moreover, p19 is recognized by antibodies against bovine GCAP1 and, similarly to other GCAPs, exhibits a Ca2+-dependent electrophoretic mobility shift.
Physical description
  • Department of Biogenic Amines, Polish Academy of Sciences, Łódź, Poland
  • Department of Biogenic Amines, Polish Academy of Sciences, Łódź, Poland
  • Laboratory of Signaling Proteins, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland
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