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2002 | 49 | 4 | 899-905
Article title

p19 detected in the rat retina and pineal gland is a guanylyl cyclase-activating protein (GCAP).

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Abstracts
EN
The Ca2+-dependent activation of retina-specific guanylyl cyclase (retGC) is mediated by guanylyl cyclase-activating proteins (GCAPs). Here we report for the first time detection of a 19 kDa protein (p19) with GCAP properties in extracts of rat retina and pineal gland. Both extracts stimulate synthesis of cGMP in rod outer segment (ROS) membranes at low (30 nM) but not at high (1 mM) concentrations of Ca2+. At low Ca2+, immunoaffinity purified p19 activates guanylyl cyclase(s) in bovine ROS and rat retinal membranes. Moreover, p19 is recognized by antibodies against bovine GCAP1 and, similarly to other GCAPs, exhibits a Ca2+-dependent electrophoretic mobility shift.
Publisher

Year
Volume
49
Issue
4
Pages
899-905
Physical description
Dates
published
2002
received
2002-11-19
revised
2002-11-30
accepted
2002-12-2
Contributors
  • Department of Biogenic Amines, Polish Academy of Sciences, Łódź, Poland
  • Department of Biogenic Amines, Polish Academy of Sciences, Łódź, Poland
  • Laboratory of Signaling Proteins, L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland
References
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Document Type
Publication order reference
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YADDA identifier
bwmeta1.element.bwnjournal-article-abpv49i4p899kz
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