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2002 | 49 | 4 | 891-897
Article title

Hydrolysis of cyclic GMP in rat peritoneal macrophages.

Content
Title variants
Languages of publication
EN
Abstracts
EN
Intact rat peritoneal macrophages (rPM) treated with 3-isobutyl-1-methylxanthine (IBMX), an inhibitor of phosphodiesterases (PDEs), accumulated more cGMP than untreated cells. A PDE activity toward [3H]cGMP was detected in the soluble and particulate fractions of rPM. The hydrolysis of cGMP was Ca2+/calmodulin-independent but increased in the presence of cGMP excess. Similar results were obtained when [3H]cAMP was used as a substrate. The hydrolytic activity towards both nucleotides was inhibited in the presence of IBMX. Therefore, the PDEs of families 2, 5, 10 and 11 are potential candidates for cGMP hydrolysis in the rPM. They may not only regulate the cGMP level in a feedback-controlled way but also link cGMP-dependent pathways with those regulated by cAMP.
Year
Volume
49
Issue
4
Pages
891-897
Physical description
Dates
published
2002
received
2002-10-19
revised
2002-11-20
accepted
2002-11-21
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv49i4p891kz
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