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2002 | 49 | 4 | 823-828
Article title

Actin in human colon adenocarcinoma cells with different metastatic potential.

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Four human colon adenocarcinoma cell line variants with different metastatic potential were used to examine whether a correlation exists between actin level, state of actin polymerization and invasiveness of tumour cells. Monomeric (G), total (T) and filamentous (F) actin were determined in the cytosolic fraction of these cells. A statistically significant decrease in G actin level and increase in the state of actin polymerization (measured by F:G actin ratio) were found in the cytosol of three cell variants with higher metastatic potential and invasiveness (EB3, 3LNLN, 5W) compared with the parental cell line (LS180). Our experimental data lead to the conclusion that there is a correlation between the metastatic capacity of human colon adenocarcinoma cells and the state of actin polymerization.
Physical description
  • Department of Cell Pathology, Institute of Biochemistry and Molecular Biology, University of Wrocław, Wrocław, Poland
  • Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland
  • Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland
  • Department of Cell Pathology, Institute of Biochemistry and Molecular Biology, University of Wrocław, Wrocław, Poland
  • Ben-Ze'ev A. (1985) The cytoskeleton in cancer cells. Biochim Biophys Acta.; 780: 197-212.
  • Button E, Shapland C, Lawson D. (1995) Actin, its associated proteins and metastasis. Cell Motil Cytoskeleton; 30: 247-51.
  • Hansell EJ, Frisch SM, Tremble P, Murnane JP, Werb Z. (1995) Simian virus 40 transformation alters the actin cytoskeleton, expression of matrix metalloproteinases and inhibitors of metalloproteinases, and invasive behaviour of normal and ataxiatelangiectasia human skin fibroblasts. Biochem Cell Biol.; 73: 373-89.
  • Janmey PA, Chaponnier C. (1995) Medical aspects of the actin cytoskeleton. Curr Opin Cell Biol.; 7: 111-7.
  • Kassis J, Lauffenburger DA, Turner T, Wells A. (2001) Tumour invasion as dysregulated cell motility. Semin Cancer Biol.; 11: 105-17.
  • Katsatonis J, Tosca A, Koukouritaki SB, Theodoropoulos PA, Gravanis A, Stournaras C. (1994) Differences in the G/Total actin ratio and microfiament stability between normal and malignant human keratinocytes. Cell Biochem Funct.; 12: 267-74.
  • Kieda C, Paprocka M, Krawczenko A, Zalecki P, Dupuis P, Monsigny M, Radzikowski C, Dus D. (2002) New human microvascular endothelial cell lines with specific adhesion molecules phenotypes. Endothelium.; 9/4 (in press).
  • Le PU, Nguyen TN, Drolet-Savoie P, Leclerc N, Nabi LR. (1998) Increased beta-actin expression in an invasive Moloney sarcoma virus-transformed MDCK cell variant concentrates to the tips of multiple pseudopodia. Cancer Res.; 58: 1631-5.
  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. (1951) Protein measurement with the Folin phenol reagent. J Biol Chem.; 193: 265-75.
  • Malicka-Blaszkiewicz M . (1986) DNase-like activity and actin content in the liver of some vertebrates. Comp Biochem Physiol B.; 84 207-9.
  • Malicka-Blaszkiewicz M, Roth JS. (1981) Some factors affecting the interaction between actin in leukemia L1210 cells and DNase I. Biochem Biophys Res Commun.; 102: 594-601.
  • Malicka-Blaszkiewicz M, Styczen I, Nowak D, Hanczycowa H, Ponikowski P, Sebzda T. (1995) Actin content and polymerization in tumour, liver and serum of the hepatoma Morris 5123 tumour bearing rats. Mater Med Pol.; 27: 115-8.
  • Nowak D, Malicka-Blaszkiewicz M. (1999) Izoformy aktyny - zroznicowanie funkcji, zmiany w stanach patologicznych. Postepy Biochem.; 45: 261-9.
  • Nowak D, Majcher I, Kochman A, Malicka- Blaszkiewicz M. (1995) The changes in actin content and polymerization during hepatoma Morris 5123 tumour development. J Exp Clin Cancer Res.; 14: 37-40.
  • Opolski A, Wietrzyk J, Dus D, Kieda C, Matejuk A, Makowska A, Wojdat E, Ugorski M, Laskowska A, Klopocki A, Rygaard J, Radzikowski C. (1998) Metastatic potential and saccharide antigens expression of human colon cancer cells xenotransplanted into athymic nude mice. Folia Microbiol. (Praha); 43: 507-10.
  • Pokorna E, Jordan PW, O'Neill CH, Zicha D, Gilbert CS, Vesely P. (1994) Actin cytoskeleton and motility in rat sarcoma cell populations with different metastatic potential. Cell Motil Cytoskeleton.; 28: 25-33.
  • Shestakova EA, Wyckoff J, Jones J, Singer RH, Condeelis J. (1999) Correlation of beta-actin messenger RNA localization with metastatic potential in rat adenocarcinoma cell lines. Cancer Res.; 59: 1202-5.
  • Sheterline P, Clayton J, Sparrow JC. (1998) Protein profile. Actin. Oxford University Press, New York.
  • Stournaras C, Stiakaki E, Koukouritaki SB, Theodoropoulos PA, Kalmanti M, Fostinis Y, Gravanis A. (1996) Altered actin polymerization dynamics in various malignant cell types: evidence for differential sensitivity to cytochalasin B. Biochem Pharmacol.; 52: 1339-46.
  • Suzuki H, Nagata H, Shimada Y, Konno A. (1998) Decrease in gamma-actin expression, disruption of actin microfilaments and alterations in cell adhesion systems associated with acquisition of metastatic capacity in human salivary gland adenocarcinoma cell clones. Int J Oncol.; 12: 1079-84.
  • Verschueren H, Van Der Taelen I, Dewit J, De Braekeleer J, De Baetselier P. (1994) Metastatic competence of BW5147 T-lymphoma cell lines is correlated with in vitro invasiveness, motility and F-actin content. J Leukoc Biol.; 55: 552-6.
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