PL EN


Preferences help
enabled [disable] Abstract
Number of results
2002 | 49 | 4 | 1043-1052
Article title

Lipopolysaccharides from Mesorhizobium huakuii and Mesorhizobium ciceri: chemical and immunological comparative data.

Authors
Content
Title variants
Languages of publication
EN
Abstracts
EN
Lipopolysaccharides of two Mesorhizobium species of different host specificity were compared: M. huakuii and M. ciceri. M. huakuii sp. was represented by five strains with special consideration of M. huakuii IFO 15243T. SDS/PAGE profiles revealed that all M. huakuii LPS preparations contained low molecular mass fractions (LPS-II) of the same molecular size. All of lipopolysaccharides contained high molecular mass fractions (LPS-I). However, the high molecular mass fraction from each strain possessed an individual molecular size distribution pattern. The crossreactivity of blotted lipopolysaccharides with rabbit polyclonal antibodies against Mesorhizobium huakuii IFO 15243T whole bacteria indicated the presence of common epitope(s) within the investigated Mesorhizobium huakuii strains. Moreover, LPS from M. huakuii S52 also reacted with anti M. ciceri HAMBI 1750 serum showing that there are epitopes common for different mesorhizobial species. LPS isolated from Mesorhizobium huakuii strain IFO 15243T contained neutral sugars: L-6-deoxytalose, L-rhamnose, D-galactose and D-glucose, aminosugars: D-quinovosamine, D-glucosamine, D-2,3-diamino-2,3-dideoxyglucose and D-galacturonic and D-glucuronic acids. In the LPS preparation, fatty acids typical for Mesorhizobium strains were detected. 3-Hydroxydodecanoic, 3-hydroxy-iso-tridecanoic, 3-hydroxyeicosanoic, 3-hydroxyheneicosanoic and 3-hydroxydocosenoic acids were the major amide linked fatty acids, while iso-heptadecanoic, eicosanoic, docosenoic, as well as 27-hydroxyoctacosanoic and 27-oxooctacosanoic acids were the dominant ester linked fatty residues.
Publisher

Year
Volume
49
Issue
4
Pages
1043-1052
Physical description
Dates
published
2002
received
2002-07-09
revised
2002-10-16
accepted
2002-12-10
Contributors
author
  • Department of General Microbiology, Maria Curie-SkÅ‚odowska University, Lublin, Poland
References
  • Bhat, RU, Forsberg LS, Carlson RW. (1994) Structure of lipid A component of Rhizobium leguminosarum bv phaseoli lipopolysaccharide. J Biol Chem.; 269: 14402-10.
  • Bhat UR, Mayer H, Yokota A, Hollingsworth RI, Carlson RW. (1991) Occurrence of lipid A variants with 27-hydroxyoctacosanoic acid in lipopolysaccharides from members of the family Rhizobiaceae. J Bacteriol.; 173: 2155-9.
  • Campbell GRO, Reuhs BL, Walker GC. (2002) Chronic intracellular infection of alfalfa nodules by Sinorhizobium meliloti requires correct lipopolysaccharide core. Proc Natl Acad Sci U S A.; 99: 3938-43.
  • Carlson RW, Kalembasa S, Turowski D, Pachori P, Noel KD. (1987) Characterization of the lipopolysaccharide from a mutant of Rhizobium phaseoli which is defective in infection thread development. J Bacteriol.; 169: 4923-8.
  • Chen WX, Li GS, Qi YL, Wang ET, Li JL. (1991) Rhizobium huakuii sp. nov. isolated from the root nodules of Astragalus sinicus. Int J Syst Bacteriol.; 41: 275-80
  • Choma A, Russa R, Mayer H, Lorkiewicz Z. (1987) Chemical analysis of Azospirillum lipopolysaccharides. Arch Microbiol.; 146: 341-5.
  • Choma A, Sowinski P, Mayer H. (2000) Structute of the O-specific polysaccharide of Mesorhizobium huakuii IFO 15243T. Carbohydr Res.; 329: 459-64.
  • Choma A, Urbanik-Sypniewska T, Russa R, Kutkowska J, Mayer H. (2000a) Occurrence and taxonomic significance of oxo-fatty acids in lipopolysaccharides from members of Mesorhizobium. Syst Appl Microbiol.; 23: 185-90.
  • Gerwig GJ, Kamerling JP, Vliegenthart JFG. (1978) Determination of the D and L configuration of neutral monosaccharides by high-resolution capillary GLC. Carbohydr Res.; 62: 349-57.
  • Hisamatsu M, Nomura S, Shutsrirung A, Obata H, Teranishi K, Yamada T, Nusawantara S, Yamashita M, Murooka Y. (1997) Structural charactrization of a new acidic exopolysaccharide and cyclic (1-->2) beta-glucan produced by Rhizobium huakuii forming nodules on Astragalus sinicus. J Ferment Bioengin.; 83: 315-20
  • Jarvis BDW, Van Berkum P, Chen WX, Nour SM, Fernandez MP, Cleyet-Marel JC, Gillis M. (1997) Transfer of Rhizobium loti, Rhizobium huakuii, Rhizobium ciceri, Rhizobium mediteraneum, Rhizobium tianshanense to Mesorhizobium gen nov. Int J Syst Bacteriol.; 47: 895-8.
  • Johnson KG, Perry MB. (1976) Improved techniques for the preparation of bacterial lipopolysaccharides. Can J Microbiol.; 22: 29-34.
  • Kannenberg EL, Carlson RW. (2001) Lipid A and O-chain modifications cause Rhizobium lipopolysaccharides to become hydrophobic during bacteroid development. Mol Microbiol.; 39: 379-91.
  • Kannenberg EL, Reuhs BL, Forsberg S, Carlson RW. (1998) Lipopolysaccharides and K-antigens: their structure biosynthesis and functions. In Rhizobiaceae. Spaink HP, Kondorosi A, Hooykaas PJJ, eds, pp81-96. Kluwer Acad Publ, Dordrecht, Boston, London.
  • Laemmli UK. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature.; 227: 680-5.
  • Malek W, Sajnaga E. (1999) Current taxonomy of the Rhizobiaceae. Acta Microbiol Polon.; 2: 109-11
  • Mayer H, Krauss JH, Urbanik-Sypniewska T, Puvanesarajah V, Stacey G, Auling G. (1989) Lipid A with 2,3-diamnio-2,3-dideoxy-glucose in lipopolysaccharides from slow-growing members of Rhizobiaceae and from Pseudomonas carboxydovorans. Arch Microbiol.; 151: 111-6.
  • Nour SM, Fernandez MP, Normand P, Cleyet- Marel J-C. (1994) Rhizobium ciceri sp. no.v consisting of strains that nodulate chickpeas. ( Cicer arietinum L). Int J Syst Bacteriol.; 44: 511-22.
  • Nuswantara S, Fujie M, Sukiman HI, Yamashita M, Yamada T, Murooka Y. (1997) Phylogeny of bacterial symbionts of the leguminous tree Acacia mangium. J Ferment Bioeng.; 84: 511-8.
  • Que NLS, Lin S, Cotter RJ, Raetz CRH. (2000) Purification and mass spectrometry of six lipid A species from the bacterial endosymbiont Rhizobium etli. J Biol Chem.; 275: 28006-16.
  • Que NLS, Ribeiro AA, Raetz CRH. (2000a) Two-dimentional NMR spectroscopy and structures of six lipid A species from Rhizobium etli CE3. J Biol Chem.; 275: 28017-27.
  • Rasanen LA, Russa R, Urbanik T, Choma A, Mayer H, Lindstrm K. (1997) Characterization of two lipopolysaccharide type isolated from Rhizobium galegae. Acta Biochim Polon.; 44: 819-26.
  • Russa R, Urbanik-Sypniewska T, Shashkov AS, Kochanowski H, Mayer H. (1995) The structure of the homopolymeric O-specific chain from the phenol soluble LPS of the Rhizobium loti type strain NZP2213. Carbohydr Polymers.; 27: 299-303.
  • Russa R, Urbanik-Sypniewska T, Lindstrm K, Mayer H. (1995a) Chemical characterization of two lipopolysaccharide species isolated from Rhizobium loti, NZP2213. Arch Microbiol.; 163: 345-51.
  • Sonesson A, Jantzen E, Bryn K, Tangen T, Eng J, Zahringer U. (1994) Composition of 23-dihydroxy fatty acid-containig lipopolysaccharides from Legionella israelensis Legionella maceachernii and Legionella micdadei. Microbiology.; 140: 1261-71.
  • Tighe SW, de Lajudie P, Dipietro K, Lindstrom K, Nick G, Jarvis BDW. (2000) Analysis of cellular fatty acids and phenotypic relationship of Agrobacterium, Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium species using the Sherlock Microbial Identification System. Int J Syst Evol Microbiol; 50: 787-801.
  • Tsai CM, Frasch CE. (1982) A sensitive silver-staining for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem.; 119: 115-9.
  • Urbanik-Sypniewska T, Choma A, Kutkowska J, Kaminska T, Kandefer-Szerszen M, Russa R, Dolecka J. (2000) Cytokine inducing activities of rhizobial and mesorhzobial lipopolysaccharides of different lethal toxity. Immunobiology.; 202: 408-20.
  • Vincent M. (1970) A manual for the practical study of root-nodule bacteria. International Biological Programme Handbook no15 Blackwell, Oxford, Edinburgh.
  • Wang ET, van Berkum P, Sui XH, Beyene Chen WX, Martinez-Romero E. (1999) Diversity of rhizobia associated with Amorpha fruticosa isolated from Chinese soils and description of Mesorhizobium amorphae sp. nov. Int J Syst Bacteriol.; 49: 51-65.
  • Wollenweber HW, Rietschel ET. (1990) Analysis of lipopolysaccharide (lipid A) fatty acids. J Microbiol Methods.; 11: 195-211.
  • Yokota A, Sakane T, Ophel K, Sawada H. (1993) Further studies on the cellular fatty acid composition of Rhizobium and Agrobacterium species. Inst Ferm Osaka Res Commun.; 16: 86-94.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv49i4p1043kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.