An approach based on diffusion to study ligand-macromolecule interaction.

• Authors: Mohammad R Housaindokht , Mahmood Bahrololoom , Shirin Tarighatpoor , Ali A Mossavi-Movahedi
• Languages of publication: EN

Abstracts

EN

A new approach has been developed to study binding of a ligand to a macromolecule based on the diffusion process. In terms of the Fick's first law, the concentration of free ligand in the presence of a protein can be determined by the measurement of those ligands which are diffused out. This method is applied to the study of binding of methyl-orange to lysozyme in phosphate buffer of pH 6.2, at 30°C. The binding isotherm was determined initially, followed by application of the Hill equation to the data obtained, then binding constant and binding capacity were estimated.

Keywords

EN

binding isotherm; methyl orange; ligand-protein interaction; hen egg-white lysozyme

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2002
• Volume: 49
• Issue: 3
• Pages: 703-707

Dates

published

2002

received

2002-01-02

revised

2002-04-17

accepted

2002-05-22

Contributors

author

Mohammad R Housaindokht

• Biochemistry Laboratory, Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

author

Mahmood Bahrololoom

• Biochemistry Laboratory, Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

author

Shirin Tarighatpoor

• Biochemistry Laboratory, Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

author

Ali A Mossavi-Movahedi

• Institute of Biochemistry and Biophysics, University of Teheran, Teheran, Ir

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