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2002 | 49 | 3 | 703-707
Article title

An approach based on diffusion to study ligand-macromolecule interaction.

Content
Title variants
Languages of publication
EN
Abstracts
EN
A new approach has been developed to study binding of a ligand to a macromolecule based on the diffusion process. In terms of the Fick's first law, the concentration of free ligand in the presence of a protein can be determined by the measurement of those ligands which are diffused out. This method is applied to the study of binding of methyl-orange to lysozyme in phosphate buffer of pH 6.2, at 30°C. The binding isotherm was determined initially, followed by application of the Hill equation to the data obtained, then binding constant and binding capacity were estimated.
Year
Volume
49
Issue
3
Pages
703-707
Physical description
Dates
published
2002
received
2002-01-02
revised
2002-04-17
accepted
2002-05-22
References
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv49i3p703kz
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