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2002 | 49 | 3 | 671-682
Article title

Interaction of three Caenorhabditis elegans isoforms of translation initiation factor eIF4E with mono- and trimethylated mRNA 5' cap analogues.

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EN
Abstracts
EN
Translation initiation factor eIF4E binds the m7G cap of eukaryotic mRNAs and mediates recruitment of mRNA to the ribosome during cap-dependent translation initiation. This event is the rate-limiting step of translation and a major target for translational control. In the nematode Caenorhabditis elegans, about 70% of genes express mRNAs with an unusual cap structure containing m32,2,7G, which is poorly recognized by mammalian eIF4E. C. elegans expresses five isoforms of eIF4E (IFE-1, IFE-2, etc.). Three of these (IFE-3, IFE-4 and IFE-5) were investigated by means of spectroscopy and structural modelling based on mouse eIF4E bound to m7GDP. Intrinsic fluorescence quenching of Trp residues in the IFEs by iodide ions indicated structural differences between the apo and m7G cap bound proteins. Fluorescence quenching by selected cap analogues showed that only IFE-5 forms specific complexes with both m7G- and m32,2,7G-containing caps (Kas 2×106 M-1 to 7×106 M-1) whereas IFE-3 and IFE-4 discriminated strongly in favor of m7G-containing caps. These spectroscopic results quantitatively confirm earlier qualitative data derived from affinity chromatography. The dependence of Kas on pH indicated optimal cap binding of IFE-3, IFE-4 and IFE-5 at pH 7.2, lower by 0.4 pH units than that of eIF4E from human erythrocytes. These results provide insight into the molecular mechanism of recognition of structurally different caps by the highly homologous IFEs.
Publisher

Year
Volume
49
Issue
3
Pages
671-682
Physical description
Dates
published
2002
received
2002-06-17
revised
2002-08-12
accepted
2002-08-26
Contributors
  • Department of Physics and Biophysics, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
  • Department of Physics and Biophysics, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
  • Department of Biophysics, Institute of Experimental Physics, Warsaw University, Warszawa, Poland
  • Department of Biophysics, Institute of Experimental Physics, Warsaw University, Warszawa, Poland
  • Department of Physics and Biophysics, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
  • Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, U.S.A.
  • Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, U.S.A.
  • Department of Biophysics, Institute of Experimental Physics, Warsaw University, Warszawa, Poland
  • Department of Chemistry, Warsaw University, Warszawa, Poland
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv49i3p671kz
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