Adhesion properties of human bladder cell lines with extracellular matrix components: the role of integrins and glycosylation.

• Authors: Anna Lityńska , Małgorzata Przybyło , Ewa Pocheć , Piotr Laidler
• Languages of publication: EN

Abstracts

EN

Integrin subunits present on human bladder cells displayed heterogeneous functional specificity in adhesion to extracellular matrix proteins (ECM). The non-malignant cell line (HCV29) showed significantly higher adhesion efficiency to collagen IV, laminin (LN) and fibronectin (FN) than cancer (T24, Hu456) and v-raf transfected (BC3726) cell lines. Specific antibodies to the α2, a5 and β1 integrin subunits inhibited adhesion of the non-malignant cells, indicating these integrin participation in the adhesion to ECM proteins. In contrast, adhesion of cancer cells was not inhibited by specific antibodies to the β1 integrin subunit. Antibodies to α3 integrin increased adhesion of cancer cells to collagen, LN and FN, but also of the HCV29 line with colagen. It seems that α3 subunit plays a major role in modulation of other integrin receptors especially in cancer cells. Differences in adhesion to ECM proteins between the non-malignant and cancer cell lines in response to Gal and Fuc were not evident, except for the v-raf transfected cell line which showed a distinct about 6-fold increased adhesion to LN on addition of both saccharides. N-Acetylneuraminic acid inhibited adhesion of all cell lines to LN and FN irrespective of their malignancy.

Keywords

EN

glycosylation; integrins; bladder cell lines; adhesion

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2002
• Volume: 49
• Issue: 3
• Pages: 643-650

Dates

published

2002

received

2002-01-02

revised

2002-06-25

accepted

2002-08-01

Contributors

author

Anna Lityńska

• >Department of Animal Physiology, Institute of Zoology, Jagiellonian University, Kraków, Poland

author

Małgorzata Przybyło

• >Department of Animal Physiology, Institute of Zoology, Jagiellonian University, Kraków, Poland

author

Ewa Pocheć

• >Department of Animal Physiology, Institute of Zoology, Jagiellonian University, Kraków, Poland

author

Piotr Laidler

• Institute of Medical Biochemistry, Collegium Medicum, Jagiellonian University, Kraków, Poland

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