Cross-resistance to five glucocorticoids in childhood acute lymphoblastic and non-lymphoblastic leukemia samples tested by the MTT assay: Preliminary report.

• Authors: Jan Styczyński , Mariusz Wysocki , Robert Dębski , Walentyna Balwierz , Roma Rokicka-Milewska , Michał Matysiak , Anna Balcerska , Jerzy Kowalczyk , Jacek Wachowiak , Danuta Sońta-Jakimczyk , Alicja Chybicka
• Languages of publication: EN

Abstracts

EN

In vitro antileukemic activity of five glucocorticoids and their cross-resistance pattern in childhood acute lymphoblastic and non-lymphoblastic leukemia were determined by means of the MTT assay in 25 leukemia cell samples of childhood acute leukemias. The equivalent antileukemic concentrations of the drugs tested were: 34 μM hydrocortisone (HC), 8 μM prednisolone (PRE), 1.5 μM methylprednisolone (MPR), 0.44 μM dexamethasone (DX) and 0.22 μM betamethasone (BET). In comparison with initial ALL cell samples, the relapsed ALL group was more resistant to PRE (38-fold, p = 0.044), DX (> 34-fold, p = 0.04), MPR (38-fold), BET (45-fold) and HC (33-fold). The AML cell samples were even more resistant to: PRE (>85-fold, p=0.001), DX (> 34-fold, p = 0.004), MPR (> 69-fold, p = 0.036), BET (> 69-fold, p = 0.038) and HC (54-fold, p = 0.059) when compared with ALL on initial diagnosis. A significant cross-resistance among all the glucocorticoids used was found. Only in some individual cases the cross-resistance was less pronounced.

Keywords

EN

cross-resistance; ALL; sensitivity; acute leukemia; MTT assay; AML; glucocorticoid resistance

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2002
• Volume: 49
• Issue: 1
• Pages: 93-98

Dates

published

2002

received

2001-09-10

revised

2002-01-03

accepted

2002-02-21

Contributors

author

Jan Styczyński

• Department of Pediatric Hematology and Oncology, Medical University, Bydgoszcz, Poland

author

Mariusz Wysocki

• Department of Pediatric Hematology and Oncology, Medical University, Bydgoszcz, Poland

author

Robert Dębski

• Department of Pediatric Hematology and Oncology, Medical University, Bydgoszcz, Poland

author

Walentyna Balwierz

• Department of Pediatric Hematology, Collegium Medicum Jagiellonian University, Kraków, Poland

author

Roma Rokicka-Milewska

• Department of Pediatric Hematology and Oncology, Medical University, Warszawa, Poland

author

Michał Matysiak

• Department of Pediatric Pulmonology, Alergology and Hematology, Medical University, Warszawa, Poland

author

Anna Balcerska

• Department of Pediatric Hematology, Oncology and Endocrinology, Medical University, Gdańsk, Poland;

author

Jerzy Kowalczyk

• Department of Pediatric Hematology and Chemotherapy, Silesian Medical University, Zabrze, Poland

author

Jacek Wachowiak

• Department of Pediatric Hematology and Oncology, Medical University, Poznań, Poland

author

Danuta Sońta-Jakimczyk

• Department of Pediatric Hematology and Chemotherapy, Silesian Medical University, Zabrze, Poland

author

Alicja Chybicka

• Department of Pediatric Hematology and Oncology, Medical University, Wrocław, Poland

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