Dual effect of 2-methoxyestradiol on cell cycle events in human osteosarcoma 143B cells.

• Authors: Justyna Gołębiewska , Piotr Rozwadowski , Jan Henryk Spodnik , Narcyz Knap , Takashi Wakabayashi , Michał Woźniak
• Languages of publication: EN

Abstracts

EN

We have demonstrated for the first time that the steroid metabolite, 2-methoxyestradiol (2-ME) is a powerful growth inhibitor of human osteosarcoma 143 B cell line by pleiotropic mechanisms involving cell cycle arrest at two different points and apoptosis. The ability of 2-ME to inhibit cell cycle at the respective points has been found concentration dependent. 1 μM 2-ME inhibited cell cycle at G1 phase while 10 μM 2-ME caused G2/M cell cycle arrest. As a natural estrogen metabolite 2-ME is expected to perturb the stability of microtubules (MT) in vivo analogously to Taxol - the MT binding anticancer agent. Contrary to 2-ME, Taxol induced accumulation of osteosarcoma cells in G2/M phase of cell cycle only. The presented data strongly suggest two different mechanisms of cytotoxic action of 2-ME at the level of a single cell.

Keywords

EN

osteosarcoma 143B cells; 2-methoxyestradiol; apoptosis; cell cycle

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2002
• Volume: 49
• Issue: 1
• Pages: 59-65

Dates

published

2002

received

2001-11-13

revised

2002-01-09

accepted

2002-02-07

Contributors

author

Justyna Gołębiewska

• Student Research Group of the Department of Medical Chemistry, Medical University of Gdańsk, Gdańsk, Poland

author

Piotr Rozwadowski

• Student Research Group of the Department of Medical Chemistry, Medical University of Gdańsk, Gdańsk, Poland

author

Jan Henryk Spodnik

• Department of Anatomy and Neurobiology, Medical University of Gdańsk, Gdańsk, Poland

author

Narcyz Knap

• Department of Medical Chemistry, Medical University of Gdańsk, Gdańsk, Poland

author

Takashi Wakabayashi

• Department of Medical Chemistry, Medical University of Gdańsk, Gdańsk, Poland

author

Michał Woźniak

• Department of Medical Chemistry, Medical University of Gdańsk, Gdańsk, Poland

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