Oligodeoxyadenylate stimulates the protein kinase activity of anti-DNA sIgA from human milk.

• Authors: Yuri Kit , Elena Kuligana , Dimitry Semenov , Vladimir Richter
• Languages of publication: EN

Abstracts

EN

Preparations of anti-DNA sIgA were obtained from human milk by sequential chromatography on protein A-sepharose, DEAE-fractogel and DNA-cellulose. The influence of oligonucleotides on protein kinase activity was investigated. It was discovered that incubation of anti-DNA sIgA with oligodeoxyriboadenylate d(A)12 stimulates the phosphorylation of polypeptides of sIgA in the presence of [γ-32P]ATP. The greatest was the incorporation of 32P into the sIgA H-chains. We also demonstrated stimulation of the casein kinase activity of anti-DNA sIgA by d(A)12. The stimulation of the protein kinase activity of anti-DNA sIgA by oligoriboadenylate r(A)12 was not detected.

Keywords

EN

anti-DNA antibodies; abzymes; protein kinase activity; oligonucleotides; human milk

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2002
• Volume: 49
• Issue: 1
• Pages: 291-294

Dates

published

2002

received

2001-09-17

revised

2001-12-20

accepted

2002-02-06

Contributors

author

Yuri Kit

• Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine

author

Elena Kuligana

• Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia

author

Dimitry Semenov

• Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia

author

Vladimir Richter

• Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia

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