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2002 | 49 | 1 | 291-294
Article title

Oligodeoxyadenylate stimulates the protein kinase activity of anti-DNA sIgA from human milk.

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EN
Abstracts
EN
Preparations of anti-DNA sIgA were obtained from human milk by sequential chromatography on protein A-sepharose, DEAE-fractogel and DNA-cellulose. The influence of oligonucleotides on protein kinase activity was investigated. It was discovered that incubation of anti-DNA sIgA with oligodeoxyriboadenylate d(A)12 stimulates the phosphorylation of polypeptides of sIgA in the presence of [γ-32P]ATP. The greatest was the incorporation of 32P into the sIgA H-chains. We also demonstrated stimulation of the casein kinase activity of anti-DNA sIgA by d(A)12. The stimulation of the protein kinase activity of anti-DNA sIgA by oligoriboadenylate r(A)12 was not detected.
Publisher

Year
Volume
49
Issue
1
Pages
291-294
Physical description
Dates
published
2002
received
2001-09-17
revised
2001-12-20
accepted
2002-02-06
Contributors
author
  • Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine
  • Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia
  • Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia
  • Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv49i1p291kz
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