Various cationic carriers for in vitro transfection of tumor and endothelial cell lines.

• Authors: Barbara Zemlińska , Aleksander Sochanik , Ewa Missol-Kolka , Stanisław Szala
• Languages of publication: EN

Abstracts

EN

We compared the efficiency of in vitro DNA transfer into selected tumor and endothelial cell lines using complexes of plasmid DNA and cationic carriers: DDAB/DOPE, DC-Chol/DOPE, Arg-Chol/DOPE, Gly-Chol/DOPE, Arg-Gly-Chol/DOPE, BGTC/DOPE, and PEI. The best carriers for transfecting the majority of tested cells lines at optimized carrier-to-DNA weight ratios were PEI and BGTC/DOPE.

Keywords

EN

cationic carriers   in vitro transfection   luciferase reporter gene  

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2002
• Volume: 49
• Issue: 1
• Pages: 285-290

Dates

published

2002

received

2001-10-16

accepted

2002-02-14

References

• 1. Ledley, F.D. (1995) Hum. Gene Ther. 6, 1129-1144.
• 2. Gao, X. & Huang, L. (1995) Gene Ther. 2, 710-722.
• 3. Fife, K., Bower, M., Cooper, R.G., Stewart, L., Etheridge, C.J., Coombes, R.C., Buluwela, L. & Miller, A.D. (1998) Gene Ther. 5, 614-620.
• 4. Li, S. & Huang, L. (2000) Gene Ther. 7, 31-34.
• 5. Mountain, A. (2000) Trends Biotechnol. 18, 119-128.
• 6. Colosimo, A., Goncz, K.K., Holmes, A.R., Kunzelmann, K., Novelli, G., Malone, R.W., Bennett, M.J. & Gruenert, D.C. (2000) BioTechniques 29, 314-331.
• 7. Crook, K., Stevenson, B.J., Dubouchet, M. & Porteous, D.J. (1998) Gene Ther. 5, 137-143.
• 8. Armeanu, S., Pelisek, J., Krausz, E., Fuchs, A., Groth, D., Curth, R., Keil, O., Quilici, J., Rolland, P.H., Reszka, R. & Nikol, S. (2000) Mol. Ther. 1, 366-375.
• 9. Keogh, M.C., Chen, D., Lupu, F., Shaper, N., Schmitt, J.F., Kakkar, V.V. & Lemoine, N.R. (1997) Gene Ther. 4, 162-171.
• 10. Liu, F., Qi, H., Huang, L. & Liu, D. (1997) Gene Ther. 4, 517-523.
• 11. Caplen, N.J., Kinrade, E., Sorgi, F., Gao, X., Gruenert, D., Geddes, D., Coutelle, C., Huang, L., Alton, E.W.F.W. & Williamson, R. (1995) Gene Ther. 2, 603-613.
• 12. Wicks, I.P., Howell, M.L., Hancock, T., Kohsaka, H., Olee, T. & Carson, D.A. (1995) Hum. Gene Ther. 6, 317-323.
• 13. Horn, N.A., Meek, J.A., Budahazi, G. & Marquet, M. (1995) Hum. Gene Ther. 6, 565-573.
• 14. Gao, X. & Huang, L. (1991) Biochem. Biophys. Res. Commun. 179, 280-285.
• 15. Vigneron, J.P., Oudrhiri, N., Fauquet, M., Vergely, L., Bradley, J.C., Basseville, M., Lehn, P. & Lehn, J.M. (1996) Proc. Natl. Acad. Sci. U.S.A. 93, 9682-9686.
• 16. Sochanik, A. (1999) Ph.D. Thesis. Institute of Immunology and Experimental Therapy, Wroclaw, Poland, 20-22.
• 17. Rose, J.K., Buonocore, L. & Whitt, M.A. (1991) BioTechniques 10, 520-525.
• 18. Abdallah, B., Hassan, A., Benoist, C., Goula, D., Behr, J.P. & Demeneix, B. (1996) Hum. Gene Ther. 7, 1947-1954.
• 19. Boussif, O., Lezoualc’h, F., Zanta, M.A., Mergny, M.D., Scherman, D., Demeneix, B. & Behr, J.P. (1995) Proc. Natl. Acad. Sci. U.S.A. 92, 7297-7301.
• 20. Mahato, R.I., Takakura, Y. & Hashida, M. (1997) Crit. Rev. Ther. Drug Carrier Systems. 14, 133-172.
• 21. Brunner, S., Sauer, T., Carotta, S., Cotten, M., Saltik, M. & Wagner, E. (2000) Gene Ther. 7, 401-407.