Various cationic carriers for in vitro transfection of tumor and endothelial cell lines.

• Authors: Barbara Zemlińska , Aleksander Sochanik , Ewa Missol-Kolka , Stanisław Szala
• Languages of publication: EN

Abstracts

EN

We compared the efficiency of in vitro DNA transfer into selected tumor and endothelial cell lines using complexes of plasmid DNA and cationic carriers: DDAB/DOPE, DC-Chol/DOPE, Arg-Chol/DOPE, Gly-Chol/DOPE, Arg-Gly-Chol/DOPE, BGTC/DOPE, and PEI. The best carriers for transfecting the majority of tested cells lines at optimized carrier-to-DNA weight ratios were PEI and BGTC/DOPE.

Keywords

EN

cationic carriers; in vitro transfection; luciferase reporter gene

• Journal: Acta Biochimica Polonica
• Year: 2002
• Volume: 49
• Issue: 1
• Pages: 285-290

Dates

published

2002

received

2001-10-16

accepted

2002-02-14

Contributors

author

Barbara Zemlińska

• Oncology-Maria Skłodowska-Curie Memorial Institute, Gliwice, Poland

author

Aleksander Sochanik

• Oncology-Maria Skłodowska-Curie Memorial Institute, Gliwice, Poland

author

Ewa Missol-Kolka

• Oncology-Maria Skłodowska-Curie Memorial Institute, Gliwice, Poland

author

Stanisław Szala

• Department of Molecular Biology, Center of Oncology-Maria Skłodowska-Curie Memorial Institute, Gliwice, Poland

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