Preferences help
enabled [disable] Abstract
Number of results
2002 | 49 | 1 | 263-268
Article title

Polymorphism in intron 23 of the endothelial nitric oxide synthase gene (NOS3) is not associated with hypertension.

Title variants
Languages of publication
Nitric oxide (NO) is synthesised in the vascular endothelium by nitric oxide synthase (NOS3) and is an important factor in the regulation of blood pressure. Impaired synthesis of NO due to mutations in the NOS3 gene is associated with hypertension. To date several allelic variants of the NOS3 gene have been identified and their possible linkage with hypertension investigated. We studied the distribution of genotypes and frequency of alleles of the G11T polymorphism in intron 23 of the NOS3 gene in patients with hypertension and in a control group of healthy individuals. The polymorphism was determined by PCR-RFLP analysis. The distribution of genotypes in the patients with hypertension and in the healthy individuals did not differ significantly from the values predicted from Hardy-Weinberg equilibrium for the general population. No major differences in the distribution of the G11T polymorphism in the patients and healthy individuals were found (P > 0.05).
Physical description
  • 1. Ignarro, L.J. (1996) Physiology and pathophysiology of nitric oxide. Kidney Int.(Suppl.) 55, 2-5.
  • 2. Derebecka, N., Hołysz, M. & Trzeciak, W.H. (2000) Genes of nitric oxide synthases: Structure, regulation of expression, protein products. Postępy Biochemii 2, 130-139 (in Polish).
  • 3. Chen, P.F., Tsai, A.L., Berka, V. & Wu, K.K. (1996) Endothelial nitric oxide synthase. J. Biol. Chem. 271, 14631-14635.
  • 4. Marsden, P.A., Heng, H.H.Q., Scherer, S.W., Stewart, R.J., Hall, A.V., Shi, X.M., Tsui, L.C. & Schappert, K.T. (1993) Structure and chromosomal localization of the human constitutive endothelial nitric oxide synthase gene. J. Biol. Chem. 268, 17478-17488.
  • 5. Huang, P.L., Huang, Z., Mashimo, H., Bloch, K.D., Moskowitz, M.A., Bevan, J.A. & Fishman, M.C. (1995) Hypertension in mice lacking the gene for endothelial nitric oxide synthase. Nature. 377, 239-242.
  • 6. Wattanapitayakul, S.K., Mihm, M., Young, A.P. & Bauer, J.A. (2001) Therapeutic implication of human endothelial nitric oxide synthase gene polymorphism. Trends Pharmacol. Sci. 22, 361-368.
  • 7. Pulkkinen, A., Viitanen, L., Kareinen, A., Lehto, S., Vauhkonen, I. & Laakso, M. (2000) Intron 4 polymorphism of the endothelial nitric oxide synthase gene is associated with elevated blood pressure in type 2 diabetic patients with coronary heart disease. J. Mol. Med. 78, 372-379.
  • 8. Hingorani, A.D., Liang, C.F., Fatibene, J., Lyon, A., Monteith, S., Parsons, A., Haydock, S., Hopper, R.V., Stephens, N.G., O’Shaughnessy, K.M. & Broen, M.J. (1999) A common variant of the endothelial nitric oxide synthase (Glu298-->Asp) is major risk factor for coronary artery disease in the UK. Circulation 100, 1515-1520.
  • 9. Bonnardeaux, A., Nadaud, S., Charru, A., Jeunemaitre, X., Corvol, P. & Soubrier, F. (1995) Lack of evidence for linkage of the endothelial cell nitric oxide synthase gene to essential hypertension. Circulation. 91, 96-102.
  • 10. Miyamoto, Y., Yoshihiko, S., Kajiyama, M., Yoshimura, M., Shimasaki, Y., Nakayama, M., Kamitani, S., Harada, M., Ishikawa, M., Kuwahara, K., Ogawa, E., Hamanaka, I., Takahashi, N., Kaneshige, T., Teraoka, H., Akamizu, T., Azuma, N., Yoshimasa, Y., Yoshimasa, T., Itoh, H., Masuda, I., Yasue, H. & Nakao, K. (1998) Endothelial nitric oxide synthase gene is positively associated with essential hypertension. Hypertension. 32, 3-8.
  • 11. Nakayama, M., Yasue, H., Yoshimura, M., Shimasaki, Y., Kugiyama, K., Ogawa, H., Motoyama, T., Saito, Y., Ogawa, Y., Miyamota, Y. & Nakao, K. (1999) T-786-->C mutation in the 5'-flanking region of the endothelial nitric oxide synthase gene is associated with coronary spasm. Circulation. 99, 2864-2870.
  • 12. World Health Organization-International Society of Hypertension guidelines for the management of hypertension. Guidelines subcommittee. (1999) J. Hypertens 17, 151-183.
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.