Monitoring of urine nitric oxide (NO) related substrates and immunological competence in hematological malignancy.

• Authors: Junko Tanaka , Kunio Koshimura , Michihiro Tsumori , Yoshio Murakami , Yuzuru Kato
• Languages of publication: EN

Abstracts

EN

It has been reported that concentrations of neopterin in the urine are changed according to the host immunological conditions. In the present study, we measured urinary concentration of neopterin in patients with malignant hematological disorders and investigated the relationship between urinary neopterin levels and laboratory indices for cellular immunity. Urine neopterin levels were correlated with serum sIL-2R levels in the patients with malignant lymphoma, and inversely correlated with lymphocyte reactivity with ConA in the patients with acute myelocytic leukemia. However, no significant correlation was observed between urine neopterin levels and lymphocyte reactivity with phytohemagglutinin and pokeweed mitogen, CD4/8 ratio, CD56+16+ subset or serum IFN-β levels. In the patients with malignant lymphoma, parallel changes in serum sIL-2R and urine neopterin were observed. The presented results suggest that urine neopterin levels are related to the activation of T cells in malignant lymphoma.

Keywords

EN

pokeweed mitogen; hematological malignancy; urine nitric oxide; phytohemagglutinin

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2002
• Volume: 49
• Issue: 1
• Pages: 227-232

Dates

published

2002

received

2001-09-10

revised

2002-01-29

accepted

2002-02-12

Contributors

author

Junko Tanaka

• First Division, Department of Medicine, Shimane Medical University, Izumo, Japan

author

Kunio Koshimura

• First Division, Department of Medicine, Shimane Medical University, Izumo, Japan

author

Michihiro Tsumori

• First Division, Department of Medicine, Shimane Medical University, Izumo, Japan

author

Yoshio Murakami

• First Division, Department of Medicine, Shimane Medical University, Izumo, Japan

author

Yuzuru Kato

• First Division, Department of Medicine, Shimane Medical University, Izumo, Japan

References

• 1. Brune, B., Götz, C., Mesmer, U.K., Sandau, K., Hirvonen, M.R. & Lapetina, E.G. (1997) Superoxide formation and macrophage resistance to nitric oxide-mediated apoptosis. J. Biol. Chem. 272, 7253-7258.
• 2. Evans, T.J., Buttery, L.D.K., Carpenter, A., Springall, D.R., Polak, J.M. & Cohen, J. (1996) Cytokine-treated human neutrophils contain inducible nitric oxide synthase that produces nitration of ingested bacteria. Proc. Natl. Acad. Sci. U.S.A. 93, 9553-9558.
• 3. Filep, J.G., Baron, C., Lachance, S., Perreault, C. & Chan, J.S.D. (1996) Involvement of nitric oxide in target-cell lysis and DNA fragmentation induced by murine natural killer cells. Blood 87, 5136-5143.
• 4. Werner, E.R., Werner-Felmayer, G., Fuchs, D., Hausen, A., Reibnegger, G., Yim, J.J., Pfleiderer, W. & Wachter, H. (1990) Tetrahydrobiopterin biosynthetic activities in human macrophages, fibroblasts, THP-1, and T 24 cells. J. Biol. Chem. 265, 3189-3192.
• 5. Ziegler, I., Schott, K., Lubbert, M., Herrmann, F., Schwulera, U. & Bacher, A. (1990) Control of tetrahydrobiopterin synthesis in T lymphocytes by synergistic action of interferon-γ and interleukin-2. J. Biol. Chem. 265, 17026-17030.
• 6. Lim, K.L., Muir, K. & Powell, R.J. (1994) Urine neopterin: A new parameter for serial monitoring of disease activity in patients with systemic lupus erythematosus. Ann. Rheum. Dis. 53, 743-748.
• 7. Huber, C., Batchelor, J.R., Fuchs, D., Hausen, A., Lang, A., Niederwieser, D., Reibnegger, G., Swethy, P., Troppmair, J. & Wachter, H. (1984) Immune response-associated production of neopterin: Release from macrophages primarily under control of interferon-gamma. J. Exp. Med. 160, 310-316.
• 8. Hibiya, M., Teradaira, R., Sugimoto, T., Fujita, K. & Nagatsu, T. (1995) Simultaneous determination of N2-(3-aminopropyl)biopterin (oncopterin), biopterin and neopterin by high-performance liquid chromatography with fluorescence detection. J. Chromatogr. B Biomed Appl 672, 143- 148.
• 9. Satoh, T., Brown, L.M., Blattner, W.A., Maloney, E.M., Kurman, C.C., Nelson, D.L., Fuchs, D., Wachter, H. & Tollerud, D.J. (1998) Serum neopterin, β2-microglobulin, soluble interleukin-2 receptors, and immunoglobulin levels in healthy adolescents. Clin. Immunol. Immunopathol. 88, 176-182.
• 10. Rubin, L.A., Kurman, C.C., Fritz, M.E., Biddison, W.E., Boutin, B., Yarchoan, R. & Nelson, D.L. (1985) Soluble interleukin 2 receptors are released from activated human lymphoid cells in vitro. J. Immunol. 135, 3172-3177.